@article{Oo2022,

title = {Inclusion complexation of emodin with various β-cyclodextrin derivatives: Preparation, characterization, molecular docking, and anticancer activity},

author = {A. Oo and P. Mahalapbutr and K. Krusong and P. Liangsakul and S. Thanasansurapong and V. Reutrakul and C. Kuhakarn and P. Maitarad and A. Silsirivanit and P. Wolschann and S. Putthisen and K. Kerdpol and T. Rungrotmongkol},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85138450015&doi=10.1016%2fj.molliq.2022.120314&partnerID=40&md5=bf889009eab5e2967b8915c7e1831dba},

doi = {10.1016/j.molliq.2022.120314},

year  = {2022},

date = {2022-01-01},

journal = {Journal of Molecular Liquids},

volume = {367},

abstract = {Encapsulation with β-cyclodextrin (βCD) has primarily been used to enhance the solubility of lipophilic guest molecules. Emodin (ED) stands out for its profound biological properties, especially anticancer activity. However, its usage in pharmaceutical applications has been hindered by its poor aqueous solubility. In the present work, we performed inclusion complexation of ED with βCD and its derivatives: hydroxypropyl-β-cyclodextrin (HPβCD), sulfobutylether-β-cyclodextrin with degree of substitution (DS) of 2 (SBE2βCD) and 6.0–7.1 (SBE7βCD) and 2,6-di-O-methyl-β-cyclodextrin (DMβCD), to identify the most promising drug carrier to enhance the water solubility and the anticancer activity of ED. The AL-type phase solubility diagram indicates 1:1 stoichiometry between ED and βCDs. The ED/DMβCD complex shows the highest stability (KC = 3800 M−1) and the highest water solubility (113.86 µg/mL) at 30 °C, followed by ED/SBE7βCD, ED/HPβCD, ED/SBE2βCD, and ED/βCD complexes. The SEM micrographs and DSC thermograms confirmed the inclusion complex formation between host and guest by attaining new surface morphological structures and thermal behaviors, respectively. Molecular docking suggests C-form insertion as the preferred binding mode of ED toward βCDs. Interestingly, the anti-proliferative effect on KKU-213A, KKU-213B, A549, and H1975 cancer cell lines of inclusion complexes, especially ED/DMβCD, ED/SBE7βCD, and ED/HPβCD, was significantly higher than that of ED alone. © 2022 Elsevier B.V.},

note = {cited By 0},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Sitthiyotha2022,

title = {Computational design of Lactobacillus Acidophilus α-L-rhamnosidase to increase its structural stability},

author = {T. Sitthiyotha and M. Klaewkla and K. Krusong and R. Pichyangkura and S. Chunsrivirot},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85130967138&doi=10.1371%2fjournal.pone.0268953&partnerID=40&md5=e9645006e479db1e3a565b61fd134681},

doi = {10.1371/journal.pone.0268953},

year  = {2022},

date = {2022-01-01},

journal = {PLoS ONE},

volume = {17},

number = {5 May},

abstract = {α-L-rhamnosidase catalyzes hydrolysis of the terminal α-L-rhamnose from various natural rhamnoglycosides, including naringin and hesperidin, and has various applications such as debittering of citrus juices in the food industry and flavonoid derhamnosylation in the pharmaceutical industry. However, its activity is lost at high temperatures, limiting its usage. To improve Lactobacillus acidophilus α-L-rhamnosidase stability, we employed molecular dynamics (MD) to identify a highly flexible region, as evaluated by its root mean square fluctuation (RMSF) value, and computational protein design (Rosetta) to increase rigidity and favorable interactions of residues in highly flexible regions. MD results show that five regions have the highest flexibilities and were selected for design by Rosetta. Twenty-one designed mutants with the best ΔΔG at each position and ΔΔG < 0 REU were simulated at high temperature. Eight designed mutants with ΔRMSF of highly flexible regions lower than -10.0% were further simulated at the optimum temperature of the wild type. N88Q, N202V, G207D, Q209M, N211T and Y213K mutants were predicted to be more stable and could maintain their native structures better than the wild type due to increased hydrogen bond interactions of designed residues and their neighboring residues. These designed mutants are promising enzymes with high potential for stability improvement. © 2022 Sitthiyotha et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.},

note = {cited By 0},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Krusong2022,

title = {Production of Large-Ring Cyclodextrins by Amylomaltases},

author = {K. Krusong and A. Ismail and K. Wangpaiboon and P. Pongsawasdi},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85125174170&doi=10.3390%2fmolecules27041446&partnerID=40&md5=09577c60d505f9dcb9e58f1c49892914},

doi = {10.3390/molecules27041446},

year  = {2022},

date = {2022-01-01},

journal = {Molecules},

volume = {27},

number = {4},

abstract = {Amylomaltase is a well-known glucan transferase that can produce large ring cyclodextrins (LR-CDs) or so-called cycloamyloses via cyclization reaction. Amylomaltases have been found in several microorganisms and their optimum temperatures are generally around 60–70◦ C for thermostable amylomaltases and 30–45◦ C for the enzymes from mesophilic bacteria and plants. The optimum pHs for mesophilic amylomaltases are around pH 6.0–7.0, while the thermostable amylomaltases are generally active at more acidic conditions. Size of LR-CDs depends on the source of amylomaltases and the reaction conditions including pH, temperature, incubation time, and substrate. For example, in the case of amylomaltase from Corynebacterium glutamicum, LR-CD productions at alkaline pH or at a long incubation time favored products with a low degree of polymerization. In this review, we explore the synthesis of LR-CDs by amylomaltases, structural information of amylomaltases, as well as current applications of LR-CDs and amylomaltases. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.},

note = {cited By 1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Trithavisup2022,

title = {Molecular structure and properties of cassava-based resistant maltodextrins},

author = {K. Trithavisup and Y. -C. Shi and K. Krusong and K. Tananuwong},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85113638545&doi=10.1016%2fj.foodchem.2021.130876&partnerID=40&md5=39c37566b45b20c02c5bec39b38be932},

doi = {10.1016/j.foodchem.2021.130876},

year  = {2022},

date = {2022-01-01},

journal = {Food Chemistry},

volume = {369},

abstract = {In-depth molecular structure and properties of cassava-derived resistant maltodextrins (RMDs) were determined. Cassava starch was dextrinized with 0.04% or 0.06% HCl at 120 °C for 60–180 min to obtain resistant dextrins (RDs), which were further hydrolyzed by α-amylase to produce RMDs. Prolonging dextrinization duration decreased proportion of α-1,4 linkages and α-/β-reducing ends but increased fraction of indigestible α-/β-1,6, β-1,4, β-1,2 linkages, degree of branching (DB), degree of polymerization, relative molecular weight, and total dietary fiber (TDF) content of the RMDs. Moreover, RMDs had greater proportion of β-glycosidic linkages, α-/β-reducing end, DB, TDF, and low molecular weight dietary fiber (LMWDF) content than their RD counterparts. Potential prebiotic activity score was higher in RMDs with abundant LMWDF fraction but low DB. Slight difference in the glass transition temperature of maximally freeze-concentrated unfrozen phase (Tg’) and unfrozen water content was found among RMDs. However, RMDs had lower Tg’ than their RD counterparts. © 2021 Elsevier Ltd},

note = {cited By 0},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Sangwongchai2022370,

title = {Salt tolerance at vegetative stage is partially associated with changes in grain quality and starch physicochemical properties of rice exposed to salinity stress at reproductive stage},

author = {W. Sangwongchai and K. Krusong and M. Thitisaksakul},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85108798441&doi=10.1002%2fjsfa.11367&partnerID=40&md5=e49248f870d457387877114dde60664f},

doi = {10.1002/jsfa.11367},

year  = {2022},

date = {2022-01-01},

journal = {Journal of the Science of Food and Agriculture},

volume = {102},

number = {1},

pages = {370-382},

abstract = {BACKGROUND: Rice yield and grain quality are highly sensitive to soil salinity. Distinct rice genotypes respond to salinity stress differently. To explore the variation in grain yield and grain trait adaptation to moderate, reproductive-stage salinity stress (4 dS/m electrical conductivity), four rice cultivars differing in degrees of vegetative salt tolerance, including Pokkali (salt-tolerant), RD15 (moderately salt-tolerant), KDML105 (moderately salt-susceptible) and IR29 (salt-susceptible), were examined. RESULTS: Grain fertility and 100-grain weight of RD15, KDML105 and IR29, as well as grain morphology of KDML105 and IR29, were significantly disturbed. Interestingly, grain starch accumulation in RD15 and KDML105 was enhanced under stress. However, only RD15 showed changes in starch physicochemical properties, including increased granule diameter, decreased gelatinization peak temperature (Tp) and decreased retrogradation onset temperature (To). Notably, Pokkali maintained productivity, grain quality, and starch properties, while the grain quality of IR29 remained unchanged under salinity stress. Multivariate analysis displayed clear separation of productivity, grain morphology, and starch variables of RD15 in the salt-treated group relative to the control group, suggesting that it was the cultivar most impacted by salt stress despite its moderate salt-tolerance at vegetative stage. CONCLUSION: Our results demonstrate specific salinity responses among the rice genotypes, and suggest discrepancies between degrees of salt tolerance at vegetative stage versus the ability to maintain both grain quality and starch properties in response to salinity stress imposed at reproductive stage. © 2021 Society of Chemical Industry. © 2021 Society of Chemical Industry.},

note = {cited By 4},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Kitipaspallop2022,

title = {Developmental effects of sesamolin on zebrafish (Danio rerio) embryos},

author = {W. Kitipaspallop and S. Sillapaprayoon and P. Phuwapraisirisan and W. -K. Kim and C. Chanchao and W. Pimtong},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85125661694&doi=10.1016%2fj.cbpc.2022.109319&partnerID=40&md5=dbb8efed54bd55b4df9b398c7a2f89e9},

doi = {10.1016/j.cbpc.2022.109319},

year  = {2022},

date = {2022-01-01},

journal = {Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology},

volume = {256},

abstract = {Sesamolin is one of the major active compounds found in sesame seeds (Sesamum indicum L.) that are commonly and increasingly used as an ingredient in cuisines and various food products. The compound has been reported to have several pharmaceutical activities such as antioxidant, antimicrobial, neuroprotective, and anticancer. However, the toxicological profile of sesamolin does not currently include developmental toxicity. In this study, we assessed sesamolin toxicity to embryonic development of zebrafish by exposure for 72 h at concentrations ranging from 10 to 50 μM. The evaluation revealed that sesamolin did not affect survival and hatching rates. However, it did induce embryo malformations and reduced embryonic heart rates in a dose-dependent manner. By qRT-PCR analysis, it downregulated the expression of oxidative stress-related genes, including superoxide dismutase 1 (sod1), catalase (cat), and glutathione S-transferase pi 2 (gstp2). Alkaline phosphatase staining of embryos revealed that sesamolin inhibited the development of subintestinal vessels, and hemoglobin staining revealed a negative impact on embryonic erythropoiesis. These findings showed that sesamolin affected genes related to angiogenesis and erythropoiesis. The risks of sesamolin to embryonic development found in this study may imply similar effects in humans and other mammals. © 2021},

note = {cited By 0},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Khongkarat2022,

title = {Phytochemical content, especially spermidine derivatives, presenting antioxidant and antilipoxygenase activities in Thai bee pollens},

author = {P. Khongkarat and P. Phuwapraisirisan and C. Chanchao},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85131208964&doi=10.7717%2fpeerj.13506&partnerID=40&md5=9d90ba8bfbf0559f2546b63dea71e961},

doi = {10.7717/peerj.13506},

year  = {2022},

date = {2022-01-01},

journal = {PeerJ},

volume = {10},

abstract = {Background. Bee pollen (BP) is full of useful nutrients and phytochemicals.Its chemical components and bioactivities depend mainly on the type of floral pollen. Methods. Monofloral BP from Camellia sinensis L., Mimosa diplotricha, Helianthus annuus L., Nelumbo nucifera, Xyris complanata, and Ageratum conyzoides were harvested. Crude extraction and partition were performed to yield solvent-partitioned extracts of each BP. Total phenolic content (TPC) was assayed by the Folin-Ciocalteu method, while the flavonoid content (FC) was measured by the aluminium chloride colorimetric method. Antioxidant capacity was measured by the (i) 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, (ii) 2,2’-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) scavenging activity and its Trolox equivalent antioxidant capacity (TEAC), and (iii) ferric reducing antioxidant power (FRAP). All samples were tested for lipoxygenase inhibitory (LOXI) activity. The most active sample was enriched by silica gel 60 column chromatography (SiG60-CC) and high performance liquid chromatography (HPLC), observing the chemical pattern of each fraction using thin layer chromatography. Chemical structure of the most active compound was analyzed by proton nuclear magnetic resonance and mass spectrometry. Results. Dichloromethane (DCM)-partitioned BP extracts of H. annuus L. and M. diplotricha (DCMMBP) showed a very high TPC, while DCMMBP had the highest FC. In addition, DCMMBP had the strongest DPPH and ABTS radical scavenging activities (as a TEAC value), as well as FRAP value. Also, DCMMBP (60 µg/mL) gave the highest LOXI activity (78.60 ± 2.81%). Hence, DCMMBP was chosen for further enrichment by SiG60-CC and HPLC. Following this, the most active fraction showed higher antioxidant andLOXI activities with an EC50 for DPPH and ABTS of 54.66 ± 3.45 µg/mL and 24.56 ± 2.99 µg/mL (with a TEAC value of 2,529.69 ± 142.16 µmole TE/g), respectively, and a FRAP value of 3,466.17 ± 81.30 µmole Fe2+/g and an IC50 for LOXI activity of 12.11 ± 0.36 µg/mL. Triferuloyl spermidines were revealed to be the likely main active components. Conclusions. TPC, FC, and spermidine derivatives played an important role in the antioxidant and antilipoxygenase activities in M. diplotricha bee pollen. Copyright 2022 Khongkarat et al.},

note = {cited By 1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Khongkarat2022b,

title = {First report of fatty acids in Mimosa diplotricha bee pollen with in vitro lipase inhibitory activity},

author = {P. Khongkarat and P. Traiyasut and P. Phuwapraisirisan and C. Chanchao},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85122314169&doi=10.7717%2fpeerj.12722&partnerID=40&md5=1a073fb0abbd6f8761bf04e7cd5f3b0f},

doi = {10.7717/peerj.12722},

year  = {2022},

date = {2022-01-01},

journal = {PeerJ},

volume = {9},

abstract = {Bee pollen (BP) is full of nutrients and phytochemicals, and so it is widely used as a health food and alternative medicine. Its composition and bioactivity mainly depend on the floral pollens. In this work, BP collected by Apis mellifera with different monoculture flowering crops (BP1-6) were used. The types of floral pollen in each BP were initially identified by morphology, and subsequently confirmed using molecular phylogenetic analysis. Data from both approaches were consistent and revealed each BP to be monofloral and derived from the flowers of Camellia sinensis L., Helianthus annuus L., Mimosa diplotricha, Nelumbo nucifera, Xyris complanata, and Ageratum conyzoides for BP1 to BP6, respectively. The crude extracts of all six BPs were prepared by sequential partition with methanol, dichloromethane (DCM), and hexane. The crude extracts were then tested for the in vitro (i) α-amylase inhibitory, (ii) acetylcholinesterase inhibitory (AChEI), and (iii) porcine pancreatic lipase inhibitory (PPLI) activities in terms of the percentage enzyme inhibition and half maximum inhibitory concentration (IC50). The DCM partitioned extract of X. complanata BP (DCMXBP) had the highest active α-amylase inhibitory activity with an IC50 value of 1,792.48 ± 50.56 µg/mL. The DCM partitioned extracts of C. sinensis L. BP (DCMCBP) and M. diplotricha BP (DCMMBP) had the highest PPLI activities with an IC50 value of 458.5 ± 13.4 and 500.8 ± 24.8 µg/mL, respectively), while no crude extract showed any marked AChEI activity. Here, the in vitro PPLI activity was focused on. Unlike C. sinensis L. BP, there has been no previous report of M. diplotricha BP having PPLI activity. Hence, DCMMBP was further fractionated by silica gel 60 column chromatography, pooling fractions with the same thin layer chromatography profile. The pooled fraction of DCMMBP2-1 was found to be the most active (IC50 of 52.6 ± 3.5 µg/mL), while nuclear magnetic resonance analysis revealed the presence of unsaturated free fatty acids. Gas chromatography with flame-ionization detection analysis revealed the major fatty acids included one saturated acid (palmitic acid) and two polyunsaturated acids (linoleic and linolenic acids). In contrast, the pooled fraction of DCMMBP2-2 was inactive but pure, and was identified as naringenin, which has previously been reported to be present in M. pigra L. Thus, it can be concluded that naringenin was compound marker for Mimosa BP. The fatty acids in BP are nutritional and pose potent PPLI activity. © 2022 Khongkarat et al.},

note = {cited By 2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Ramadhan20222225,

title = {The potency of selected ethnomedicinal plants from East Kalimantan, Indonesia as antidiabetic agents and free-radical scavengers},

author = {R. Ramadhan and P. Phuwapraisirisan and R. Amirta and M. F. B. Darmawan and K. Ul-Haq and I. W. Kusuma and H. Suwito and N. Abdulgani and A. Mukhdlor and Saparwadi},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85129892413&doi=10.13057%2fbiodiv%2fd230458&partnerID=40&md5=ab8f86017c9b1f3e6489799eb00d072b},

doi = {10.13057/biodiv/d230458},

year  = {2022},

date = {2022-01-01},

journal = {Biodiversitas},

volume = {23},

number = {4},

pages = {2225-2230},

abstract = {Ten ethnomedicinal plant extracts from East Kalimantan flora, traditionally used to treat blood sugar levels and other diabetes-related diseases, were examined in vitro for their antidiabetic and free radical scavenging activities by inhibiting rat alpha-glucosidase and several free radicals such as DPPH, ABTS, and Nitric oxide respectively. Out of the ten plant species investigated for their antidiabetic activity against maltase and sucrase rat alpha-glucosidase, three exhibited the strongest α-glucosidase inhibitory activity with maltose as a substrate, namely extracts of Garcinia nervosa, Syzygium caudatilimbum, and Shorea balangeran with IC50 values of 0.046; 0.037; 0.045 mg/mL. Meanwhile, quercetin as a positive control appeared to have a comparable IC50 value. Furthermore, among the ten extracts, Syzygium caudatilimbum, Shorea balangeran, and Ixora javanica showed good inhibition against sucrase rat alpha-glucosidase. Moreover, the antioxidant test showed that the ten methanol extracts of plants from East Kalimantan have an antioxidant activity indicated by IC50 values. The present study confirms that the ethnopharmacological use of selected plants from East Kalimantan might have potential as an antidiabetic and natural antioxidant. © 2022, Society for Indonesian Biodiversity. All rights reserved.},

note = {cited By 1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Do20211597,

title = {Identification of highly potent α-glucosidase inhibitors from Garcinia schomburgkiana and molecular docking studies},

author = {T. M. L. Do and N. -M. -A. Tran and T. -H. Duong and P. Phuwapraisirisan and N. Niamnont and S. Sedlak and K. Inthanon and J. Sichaem},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85130597413&doi=10.14456%2fsjst-psu.2021.209&partnerID=40&md5=27f58aa30a927c63682ebd2b8bba0a40},

doi = {10.14456/sjst-psu.2021.209},

year  = {2021},

date = {2021-01-01},

journal = {Songklanakarin Journal of Science and Technology},

volume = {43},

number = {6},

pages = {1597-1603},

abstract = {Twenty-two compounds (1-22) were isolated from the stems and twigs of Garcinia schomburgkiana. NMR, IR, UV, and MS were used for structural elucidation, and comparisons were made with previous reports. Compound 1 exhibited the most potent α-glucosidase inhibition (IC50 0.31 ± 0.7 μM), outperforming the positive control (acarbose). Molecular docking results showed that the phenolic hydroxyl groups on the phenyl rings linked with active receptor sites on the protein in 1. By preventing the duplication of DNA sequences, Compound 1 is an excellent inhibitor of the α-glucosidase enzyme, and represents a potential novel class of α-glucosidase inhibitor. © 2021, Prince of Songkla University. All rights reserved.},

note = {cited By 0},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Wisetsai2022,

title = {Fatty acid acylated flavonol glycosides from the seeds of Nephelium lappaceum and their nitric oxide suppression activity},

author = {A. Wisetsai and S. Choodej and N. Ngamrojanavanich and K. Pudhom},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85131422253&doi=10.1016%2fj.phytochem.2022.113262&partnerID=40&md5=b1aee794d1fd8f755a244b50949d2ba9},

doi = {10.1016/j.phytochem.2022.113262},

year  = {2022},

date = {2022-01-01},

journal = {Phytochemistry},

volume = {201},

abstract = {Five undescribed fatty acid esters of flavonol glycosides, nephelosides A−E, along with eight known compounds, were isolated from the seeds of Nephelium lappaceum L. The structures were elucidated by extensive analysis of spectroscopic data in combination with GC-MS analysis. Potency of compounds toward nitric oxide suppression was assessed by monitoring the inhibition of lipopolysaccharide-stimulated nitric oxide production in J744.A1 macrophage cells. Nepheloside D, kaempferol and kaempferol 7-O-α-L-rhamnopyranoside showed significant activity with IC50 values of 26.5, 11.6 and 12.0 μM, respectively. © 2022},

note = {cited By 0},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Hanpanich2022,

title = {Label-free detection of HPV mRNA with an artificial chaperone-enhanced MNAzyme (ACEzyme)-based electrochemical sensor},

author = {O. Hanpanich and A. Lomae and A. Maruyama and T. Palaga and O. Chailapakul and N. Ngamrojanavanich},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85132341833&doi=10.1016%2fj.bios.2022.114352&partnerID=40&md5=922cdd2a513cd3b9833bf01175047b4d},

doi = {10.1016/j.bios.2022.114352},

year  = {2022},

date = {2022-01-01},

journal = {Biosensors and Bioelectronics},

abstract = {Nucleic acid biosensors for point-of-care (POC) diagnostic applications are highly desirable. The ability to detect DNA and RNA in a simple, rapid, affordable and portable format leads to a range of important applications for early screening in the field of disease monitoring and management. Herein, we report the development of an isothermal, label-free electrochemical biosensor that was designed on the basis of target-driven MNAzyme cleavage activity. Hybridization with HPV mRNA, a model nucleic acid target, activated MNAzyme and initiated the cleavage of immobilized hairpin substrates, leading to changes in the electrochemical signal. Under optimal conditions, a detection limit of 2.6 pM was obtained with an incubation time of 60 min. Furthermore, an artificial chaperone-enhanced MNAzyme (ACEzyme) system was integrated to an electrochemical biosensor for the first time. The analytical performance of the biosensor was enhanced, and the detection time was significantly reduced by the addition of PLL-g-Dex, which exhibits nucleic acid chaperone-like activity. A detection limit of 0.88 pM was obtained with a threefold decrease in incubation time without prior amplification. The proposed biosensing platform shows the advantages of simple fabrication and operation, good selectivity in the presence of single-base mismatch, and excellent versatility in a complex mixture of total RNA. We believe that this isothermal, label-free, and protein-free nucleic acid analysis platform could provide foundations for the further development of a universal nucleic acid biosensing platform for clinical application. © 2022 Elsevier B.V.},

note = {cited By 0},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Jampasa2021,

title = {Ultrasensitive electrochemiluminescence sensor based on nitrogen-decorated carbon dots for Listeria monocytogenes determination using a screen-printed carbon electrode},

author = {S. Jampasa and N. Ngamrojanavanich and S. Rengpipat and O. Chailapakul and K. Kalcher and S. Chaiyo},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85106370426&doi=10.1016%2fj.bios.2021.113323&partnerID=40&md5=ea702855d17c1ef179b2de0e82bf2f56},

doi = {10.1016/j.bios.2021.113323},

year  = {2021},

date = {2021-01-01},

journal = {Biosensors and Bioelectronics},

volume = {188},

abstract = {Current method for identification of foodborne pathogens suffers from its relatively poor performance, consequently limiting its use. Herein, we first describe an ultrasensitive electrochemiluminescence (ECL) sensor based on nitrogen-decorated carbon dots (NCDs) for Listeria monocytogenes (L. monocytogenes) determination using a screen-printed carbon electrode (SPCE). Citric acid serves as carbon source, and ethylenediamine, a molecule containing nitrogen atom, is employed to synthesize CDs. Approximately 4 nm NCD with homogenous size distribution can be produced via a single step green microwave-assisted methodology. The construction of ECL sensor is initiated by the immobilization of capture antibody (Ab1) onto the carboxyl graphene (GOOH)-modified SPCE, where immunocomplexes (antigen and the NCD-labelled secondary antibody (Ab2-NCD)) are formed, resulting in a substantial increment in the ECL signal response in the presence of K2S2O8. The GOOH allows direct formation of the capture antibodies and enhances the electrochemical properties. Under optimal parameters, this sensor exhibits wide linearity (2 to 1.0 × 106 CFU mL−1), high sensitivity (0.104 or 1.0 × 10−1 CFU mL−1) and specificity over the nontargeting studied pathogens and is successfully applied to determine L. monocytogenes in food products. These promising results together with its performance suggest that this proposed platform may serve as an alternative device to effectively control the spread of foodborne diseases. © 2021 Elsevier B.V.},

note = {cited By 17},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Khumploy2021967,

title = {Picrotoxane sesquiterpene and α-pyrone derivative from Dendrobium signatum and their free radical scavenging potency},

author = {P. Khumploy and A. Raksat and S. Choodej and T. Aree and N. Ngamrojanavanich and K. Pudhom},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85113603041&doi=10.1007%2fs11418-021-01547-5&partnerID=40&md5=3a6e64aed09d26225f3d416f09fbfeec},

doi = {10.1007/s11418-021-01547-5},

year  = {2021},

date = {2021-01-01},

journal = {Journal of Natural Medicines},

volume = {75},

number = {4},

pages = {967-974},

abstract = {One new picrotoxane sesquiterpene (1) and one new α-pyrone derivative (4), together with nine known compounds, were isolated from the aerial parts of Dendrobium signatum. The structures of the new compounds were elucidated based on the interpretation of 1D and 2D NMR, HRESIMS and electronic circular dichroism (ECD) data. The absolute configuration of 1 was confirmed by single-crystal X-ray diffraction data. The new α-pyrone 4 exhibited promising ABTS scavenging activity with IC50 value of 0.7 μM. Graphic abstract: [Figure not available: see fulltext.] © 2021, The Japanese Society of Pharmacognosy.},

note = {cited By 0},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Teengam2021,

title = {NFC-enabling smartphone-based portable amperometric immunosensor for hepatitis B virus detection},

author = {P. Teengam and W. Siangproh and S. Tontisirin and A. Jiraseree-amornkun and N. Chuaypen and P. Tangkijvanich and C. S. Henry and N. Ngamrojanavanich and O. Chailapakul},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85090715876&doi=10.1016%2fj.snb.2020.128825&partnerID=40&md5=96b6249eb1d7fa5bd3e67e967ec179f0},

doi = {10.1016/j.snb.2020.128825},

year  = {2021},

date = {2021-01-01},

journal = {Sensors and Actuators, B: Chemical},

volume = {326},

abstract = {A smartphone-controlled electrochemical sensor operated entirely via Near Field Communication (NFC) was used to create a simple label-free immunoassay for detecting Hepatitis B Virus (HBV). The completed system was composed of a card-sized electrochemical NFC tag sensor integrated with a smartphone and antibody-modified electrode sensor. Screen-printed graphene electrodes (SPGE) were modified with gold nanoparticles, increasing high sensitivity while β-cyclodextrin (β-CD) was electropolymerized on the surface to capture antibodies. The modification and immobilization processes were verified by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). The NFC-based electrochemical immunosensor was then used to quantify Hepatitis B surface antigen (HBsAg) using amperometric detection by measuring the current from the (Fe(CN)6)3−/4− redox couple before and after addition of HBsAg. The linear calibration curve and limit of detection for HBsAg were found to be 10−200 μg/mL and 0.17 μg/mL, respectively. The sensor was then tested with chronic HBV infected serum samples and the system showed a good correlation with traditional immunoassays. This electrochemical immunosensor has the potential to be an alternative platform for portable, simple, sensitive and selective tools that can readily assess a variety of health indicators using a low-cost sensor system operated from a smartphone. © 2020 Elsevier B.V.},

note = {cited By 38},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{AbuBakar2022,

title = {Characterization of asiaticoside concentration, total phenolic compounds, and antioxidant activity of different varieties of Centella asiatica (L.) and essential oil extraction using hydro-distillation with enzyme assisted},

author = {I. N. Abu Bakar and M. F. Ibrahim and M. Hakiman and S. Abd-Aziz and S. Prasongsuk and L. C. Yen Tin and M. A. Jenol},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85137092151&doi=10.1016%2fj.bcab.2022.102474&partnerID=40&md5=76e3954f98e3418f18592b55c0a5ada4},

doi = {10.1016/j.bcab.2022.102474},

year  = {2022},

date = {2022-01-01},

journal = {Biocatalysis and Agricultural Biotechnology},

volume = {44},

abstract = {Centella asiatica (L.) or known locally as Pegaga has been recognized by Ministry of Agriculture (MoA) Malaysia as one of the top 10 the best herbs in Malaysia. Considering its beneficial advantages, the varieties of C. asiatica in Malaysia have been compared and further investigated for its potential to produce essential oil. In this study, the bioactive compounds, total phenolic content (TPC) and antioxidants activity from different varieties of Centella asiatica (L.) known as Pegaga Kampung, Pegaga Nyonya and Pegaga Salad from Malaysia and Pegaga Thailand have been determined followed by the extraction of essential oil using hydro-distillation (HD) and hydro-distillation with enzyme assisted (HDEA). Findings showed that Pegaga Kampung had the highest asiaticoside content of 0.259 mg/g, TPC of 9.22 mg GAE/g and DPPH radical scavenging activity (DPPH*) of 76.88% in leaf, 67.77% in petiole, and 60.37% in root as compared to other varieties of C. asiatica. Extraction of essential oils from Pegaga Kampung using HDEA with 40 FPU/gsubstrate of cellulase produced the highest yield of 1.01%. A higher TPC of 107.05 mg GAE/100 g was detected in essential oils extracted using HDEA with 10 FPU/gsubstrate of cellulase, whereas the highest DPPH* of 19.02% was found in essential oil using HD. © 2022},

note = {cited By 0},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Wahyuni20224272,

title = {Morpho-anatomical characterization and DNA barcoding analysis of Pluchea indica (L.) Less.},

author = {D. K. Wahyuni and S. R. Mukarromah and P. Rakhmad and M. Ilham and G. A. Rakashiwi and D. T. Indriati and B. F. Yoku and H. Purnobasuki and Junairiah and S. Prasongsuk},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85136778709&doi=10.13057%2fbiodiv%2fd230851&partnerID=40&md5=3b57f8d494e5678fba42026cb9aacb80},

doi = {10.13057/biodiv/d230851},

year  = {2022},

date = {2022-01-01},

journal = {Biodiversitas},

volume = {23},

number = {8},

pages = {4272-4282},

abstract = {Pluchea indica (L.) Less. is a member of the Asteraceae family and is popular as a medicinal plant. It is very important to authenticate plants using various markers to confirm the labeling of plants and to reveal the bioactive compounds for pharmaceutical research. This study aims to characterize the morpho-anatomical and DNA barcoding aspects of P. indica. Three samples obtained from Taman Husada Graha Famili, Surabaya, Indonesia were used in this study. Morphological studies were carried out descriptively, while the paraffin method was used for anatomical observations. DNA barcoding analysis was performed by amplifying and aligning the rbcL and matK genes. Morphologically, P. indica has organs like Asteraceae plants in general, with specific characteristics in leaves and flowers. P. indica has an anatomical organ similar to that of Asteraceae plants globally, only that there are specific histological features on its leaves, including anomocytic stomata, glandular cells, and multicellular glandular trichomes. Alignment and reconstruction of the phylogenetic tree showed that the sample plants were closely related to P. indica in the GenBank database. Based on morpho-anatomical characters and DNA barcodes, the plant under study was confirmed as P. indica L. (Less). © 2022, Society for Indonesian Biodiversity. All rights reserved.},

note = {cited By 0},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Ilham20221958,

title = {Morpho-anatomical characterization and DNA barcoding of Achillea millefolium L.},

author = {M. Ilham and S. R. Mukarromah and G. A. Rakashiwi and D. T. Indriati and B. F. Yoku and P. R. Purnama and Junairiah and S. Prasongsuk and H. Purnobasuki and D. K. Wahyuni},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85128731630&doi=10.13057%2fbiodiv%2fd230430&partnerID=40&md5=c295e921e76d7df4e764d1a9aa753f9d},

doi = {10.13057/biodiv/d230430},

year  = {2022},

date = {2022-01-01},

journal = {Biodiversitas},

volume = {23},

number = {4},

pages = {1958-1969},

abstract = {Ilham M, Mukarromah SR, Rakashiwi GA, Indriati DT, Yoku BF, Purnama PR, Junairiah, Prasongsuk S, Purnobasuki H, Wahyuni DK. 2022. Morpho-anatomical characterization and DNA barcoding of Achillea millefolium L. Biodiversitas 23: 1958-1969. One of the important things to study the distribution of secondary metabolites in the plant body is to carry out the identifi cation process. Morphological markers have several limitations to recognize plants, therefore supporting data is needed so that the information becomes more comprehensive. This study aims to identify Achillea millefolium L. based on morphological, anatomical, and DNA barcoding markers to obtain specific data and avoid confusion. Morphological studies were carried out descriptively using vegetative organs, while anatomical studies of rhizomes, stems, and leaves used the paraffin method. The DNA barcoding was performed by analyzing genes from 3 different individuals. The research was carried out by amplifying and sequencing the partial gene in the ribulose-bisphosphate carboxylase large subunit (rbcL) regions and maturase-K (matK). The results showed that the plant had taproots, short stems due to root rosette and the leaves were double compound. The rhizome and stem tissues had almost the same structure, while the leaves had a tissue arrangement that was similar to other plants in general, except that the mesophyll tissue was undifferentiated. The results of DNA barcoding showed a percentage of identity above 98% for both the rbcL and matK genes. © 2022, Society for Indonesian Biodiversity. All rights reserved.},

note = {cited By 2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Ibrahim2022,

title = {Production and characterization of thermostable acidophilic β-mannanase from Aureobasidium pullulans NRRL 58524 and its potential in mannooligosaccharide production from spent coffee ground galactomannan},

author = {S. N. M. M. Ibrahim and W. Bankeeree and S. Prasongsuk and H. Punnapayak and P. Lotrakul},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85136473497&doi=10.1007%2fs13205-022-03301-4&partnerID=40&md5=453bcd68945062301a83bf13cfd5a2cc},

doi = {10.1007/s13205-022-03301-4},

year  = {2022},

date = {2022-01-01},

journal = {3 Biotech},

volume = {12},

number = {9},

abstract = {The maximum yield of the crude mannanase from Aureobasidium pullulans NRRL 58524 was 8.42 ± 0.18 U mL−1 when cultured for 72 h in the optimized medium containing 3% (w v−1) defatted spent coffee grounds (SCG) and 0.67% (w v−1) ammonium sulphate. Two forms of mannanase were observed in the crude enzyme and the principal mannanase was enriched to apparent homogeneity via sequential filtration and anion exchange chromatography. The molecular mass of the enzyme was approximately 63 kDa as determined by SDS–PAGE. The enriched mannanase was active at high temperatures (45–75 °C) and a pH range from 3 to 6 with the maximum activity at 55 °C and pH 4.0. The enzyme was relatively thermostable with more than 75% of its initial activity remained after a 12 h incubation at 55 °C. The half-lives of the enriched mannanase were over 8 and 6 h at 60 and 65 °C, respectively. The enzyme was not adversely affected by chelator and most ions tested. This enzyme could hydrolyze both glucomannan and galactomannan and exhibited limited catalytic activity on beta-glucan. When the crude mannanase was used to hydrolyze galactomannan extracted from SCG, the maximum yield of reducing sugars mainly comprising of mannobiose (16.27 ± 0.84 mg 100 mg−1), and mannotriose (2.85 ± 0.20 mg 100 mg−1) was obtained at 58.22 ± 2.04 mg 100 mg−1 dry weight, under optimized condition (84.87 U g−1 mannanase, 41 h 34 min incubation at 55 °C and pH 4.0). These results suggested the prospect of the enzyme in mannan hydrolysis and mannooligosaccharide production at a larger scale. © 2022, King Abdulaziz City for Science and Technology.},

note = {cited By 0},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Bankeeree20222677,

title = {Alkyl β-D-xyloside synthesis from black liquor xylan using Aureobasidium pullulans CBS 135684 β-xylosidases immobilized on spent expanded perlite},

author = {W. Bankeeree and T. Watanabe and H. Punnapayak and P. Lotrakul and S. Prasongsuk and R. Li and D. H. Y. Yanto},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85085510280&doi=10.1007%2fs13399-020-00755-5&partnerID=40&md5=1a94730d047eaf3b9268a8cd2d3b29cd},

doi = {10.1007/s13399-020-00755-5},

year  = {2022},

date = {2022-01-01},

journal = {Biomass Conversion and Biorefinery},

volume = {12},

number = {7},

pages = {2677-2686},

abstract = {The crude β-xylosidase from Aureobasidium pullulans CBS 135684 was immobilized on the surface of the modified spent expanded perlite via (3-aminopropyl) triethoxy-silane and glutaraldehyde cross-linking reaction. Over 80% of the initial free enzyme activity was detected on the bound enzyme. The immobilized β-xylosidase (204.7 ± 6.5 U g-1 of perlite) exhibited transxylosylation activity when sugars from black liquor xylan hydrolysate and a variety of alcohols (C1-6) were used as donor and acceptor molecules, respectively, yielding a number of alkyl xylosides. The synthesized hexyl xyloside exhibited some interesting properties comparable with those of a commercial hexyl glucoside, including an antioxidant activity (IC50 = 8.9 ± 2.0 mg mL-1), wetting time (43 s), and critical micelle concentration value (252 mmol L-1). Optimization for hexyl xyloside synthesis was performed via statistical analysis using a Box and Behnken design. The maximum predicted yield of hexyl xyloside (499.0 ± 1.1 mg g-1 xylan hydrolysate) was obtained with 10% (w/v) xylan hydrolysate, 10 U g-1 immobilized β-xylosidase, and 13% (v/v) hexanol at 70 °C, pH 6.0 for 4 h, and it was validated by the following experimental yield (498.9 ± 4.8 mg g-1 xylan hydrolysate). The maximum production yield remained relatively stable after the immobilized enzyme was reused for 12 cycles with a 471.5 ± 15.1 mg g-1 hexyl xyloside yield (94.4% of the first cycle). Thus, the immobilized β-xylosidase has a high potential as a robust catalyst for application in alkyl xyloside production. © 2020, Springer-Verlag GmbH Germany, part of Springer Nature.},

note = {cited By 1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Sukkasam20221253,

title = {Chemicals Affecting Cyanobacterial Poly(3-hydroxybutyrate) Accumulation: 2-Phenylethanol Treatment Combined with Nitrogen Deprivation Synergistically Enhanced Poly(3-hydroxybutyrate) Storage in Synechocystis sp. PCC6803 and Anabaena sp. TISTR8076},

author = {N. Sukkasam and A. Incharoensakdi and T. Monshupanee},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85138444704&doi=10.1093%2fpcp%2fpcac100&partnerID=40&md5=e58c8f76d4e7875f5f23e15dddb8c8fd},

doi = {10.1093/pcp/pcac100},

year  = {2022},

date = {2022-01-01},

journal = {Plant & cell physiology},

volume = {63},

number = {9},

pages = {1253-1272},

abstract = {Various photoautotrophic cyanobacteria increase the accumulation of bioplastic poly(3-hydroxybutyrate) (PHB) under nitrogen deprivation (-N) for energy storage. Several metabolic engineering enhanced cyanobacterial PHB accumulation, but these strategies are not applicable in non-gene-transformable strains. Alternatively, stimulating PHB levels by chemical exposure is desirable because it might be applied to various cyanobacterial strains. However, the study of such chemicals is still limited. Here, 19 compounds previously reported to affect bacterial cellular processes were evaluated for their effect on PHB accumulation in Synechocystis sp. PCC6803, where 3-(3,4-dichlorophenyl)-1,1-dimethylurea, methyl viologen, arsenite, phenoxyethanol and 2-phenylethanol were found to increase PHB accumulation. When cultivated with optimal nitrate supply, Synechocystis contained less than 0.5% [w/w dry weight (DW)] PHB, while cultivation under -N conditions increased the PHB content to 7% (w/w DW). Interestingly, the -N cultivation combined with 2-phenylethanol exposure reduced the Synechocystis protein content by 27% (w/w DW) but significantly increased PHB levels up to 33% (w/w DW), the highest ever reported photoautotrophic cyanobacterial PHB accumulation in a wild-type strain. Results from transcriptomic and metabolomic analysis suggested that under 2-phenylethanol treatment, Synechocystis proteins were degraded to amino acids, which might be subsequently utilized as the source of carbon and energy for PHB biosynthesis. 2-Phenylethanol treatment also increased the levels of metabolites required for Synechocystis PHB synthesis (acetyl-CoA, acetoacetyl-CoA, 3-hydroxybutyryl-CoA and NADPH). Additionally, under -N, the exposure to phenoxyethanol and 2-phenylethanol increased the PHB levels of Anabaena sp. from 0.4% to 4.1% and 6.6% (w/w DW), respectively. The chemicals identified in this study might be applicable for enhancing PHB accumulation in other cyanobacteria. © The Author(s) 2022. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.},

note = {cited By 0},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Kaewbai-ngam20221983,

title = {Production of glycogen, PHB, biohydrogen, NAD(P)H, and proteins in Synechocystis sp. PCC 6803 disrupted in metabolically linked biosynthetic pathway(s)},

author = {J. Kaewbai-ngam and N. Sukkasam and O. Phoraksa and A. Incharoensakdi and T. Monshupanee},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85130425845&doi=10.1007%2fs10811-022-02759-2&partnerID=40&md5=a809469f54de6ed357dd0f37c2f2c3c4},

doi = {10.1007/s10811-022-02759-2},

year  = {2022},

date = {2022-01-01},

journal = {Journal of Applied Phycology},

volume = {34},

number = {4},

pages = {1983-1995},

abstract = {Under nitrogen deprivation (-N), cell growth and protein synthesis of Synechocystis sp. PCC 6803 were inhibited but production of glycogen (GL) and poly-3-hydroxybutyrate (PHB) was enhanced, indicating the importance of -N for increasing the production of such bioproducts. Upon transition from N-deprived to N-supplied medium, GL and PHB were utilized for cell growth recovery. Here, we systematically disrupted the biosynthesis of GL, PHB and/or H2 and examined changes on amount of the remaining bioproducts. Disruption of PHB synthesis increased H2 evolution rate up to 1.7-fold under -N. Disruption of GL synthesis increased PHB level up to 1.4-fold, but did not affect H2 production under -N. Cellular NAD(P)H was elevated 1.6-fold after the disruption of GL synthesis, and by 3.6-fold after the disruption of both GL and PHB synthesis under -N. The double disruption of GL and PHB, GL and H2, or PHB and H2, significantly affected the initial (day 0–4) growth rate upon switching from -N to nitrogen repletion (+N). Under -N to +N condition at day 0–4, the disrupted synthesis of both GL and PHB significantly decreased the levels of total proteins, phycobilins, carotenoids, and chlorophyll a by 32%, 44%, 47%, and 59%, respectively. Thus, both PHB and GL storage are likely required for normal growth, as well as for the maximal production of proteins and photosynthetic pigments upon growth recovery under nitrogen repletion. The results demonstrated that the cyanobacterial production of GL, PHB, H2, NAD(P)H, and proteins can be affected by the disruption of metabolically connected biosynthetic pathway(s). © 2022, The Author(s), under exclusive licence to Springer Nature B.V.},

note = {cited By 0},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Sukkasam2022135,

title = {Disruption of Hydrogen Gas Synthesis Enhances the Cellular Levels of NAD(P)H, Glycogen, Poly(3-hydroxybutyrate) and Photosynthetic Pigments under Specific Nutrient Condition(s) in Cyanobacterium Synechocystis sp. PCC 6803},

author = {N. Sukkasam and A. Incharoensakdi and T. Monshupanee},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85123878265&doi=10.1093%2fpcp%2fpcab156&partnerID=40&md5=bd343130841d36ea33f4616da0126a94},

doi = {10.1093/pcp/pcab156},

year  = {2022},

date = {2022-01-01},

journal = {Plant and Cell Physiology},

volume = {63},

number = {1},

pages = {135-147},

abstract = {In photoautotrophic Synechocystis sp. PCC 6803, NADPH is generated from photosynthesis and utilized in various metabolism, including the biosynthesis of glyceraldehyde 3-phosphate (the upstream substrate for carbon metabolism), poly(3-hydroxybutyrate) (PHB), photosynthetic pigments, and hydrogen gas (H2). Redirecting NADPH flow from one biosynthesis pathway to another has yet to be studied. Synechocystis's H2 synthesis, one of the pathways consuming NAD(P)H, was disrupted by the inactivation of hoxY and hoxH genes encoding the two catalytic subunits of hydrogenase. Such inactivation with a complete disruption of H2 synthesis led to 1.4-, 1.9-, and 2.1-fold increased cellular NAD(P)H levels when cells were cultured in normal medium (BG11), the medium without nitrate (-N), and the medium without phosphate (-P), respectively. After 49-52 d of cultivation in BG11 (when the nitrogen source in the media was depleted), the cells with disrupted H2 synthesis had 1.3-fold increased glycogen level compared to wild type of 83-85% (w/w dry weight), the highest level reported for cyanobacterial glycogen. The increased glycogen content observed by transmission electron microscopy was correlated with the increased levels of glucose 6-phosphate and glucose 1-phosphate, the two substrates in glycogen synthesis. Disrupted H2 synthesis also enhanced PHB accumulation up to 1.4-fold under -P and 1.6-fold under -N and increased levels of photosynthetic pigments (chlorophyll a, phycocyanin, and allophycocyanin) by 1.3- to 1.5-fold under BG11. Thus, disrupted H2 synthesis increased levels of NAD(P)H, which may be utilized for the biosynthesis of glycogen, PHB, and pigments. This strategy might be applicable for enhancing other biosynthetic pathways that utilize NAD(P)H. © The Author(s) 2021. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.},

note = {cited By 2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Itthirit20213697,

title = {Efficient conversion of acetate or glucose to poly(3-hydroxybutyrate) and glycogen by the single-stage photoheterotrophic cultivation of cyanobacterium Chroococcus hansgirgi TISTR 8561},

author = {P. Itthirit and A. Incharoensakdi and T. Monshupanee},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85113412097&doi=10.1007%2fs10811-021-02578-x&partnerID=40&md5=ff5de0bf194e9ee398aff243e6fe85c0},

doi = {10.1007/s10811-021-02578-x},

year  = {2021},

date = {2021-01-01},

journal = {Journal of Applied Phycology},

volume = {33},

number = {6},

pages = {3697-3708},

abstract = {Microbial poly(3-hydroxybutyrate) (PHB) has been used as bioplastic, while the biopolymer of glucose (glycogen) can be used as efficient substrate for the generation of biofuels. Substantial production of PHB and glycogen by heterotrophic microbes has been constrained by the limited resources of organic substrates. Hence, the heterotrophic cultivation that efficiently converts an organic substrate to the two biopolymers is desirable. However, limited research has been conducted to examine the conversion efficiency of an organic substrate to biopolymers by photoheterotrophic microbes. In this study, the single-stage cultivation of a photoheterotrophic cyanobacterium (Chroococcus hansgirgi TISTR 8561) which accumulates PHB and glycogen was optimized and evaluated for the mass conversion efficiency (CE) of an organic substrate to the two biopolymers. The cells were cultured in BG11 trace element medium with addition of only a single specific organic substrate under light to enable both cellular oxygenic photosynthesis and heterotrophic metabolism, which were evidenced by the oxygen gas evolution and the organic substrate utilization of the cells, respectively. Through optimization of the culture conditions, organic substrate supply, and cultivation time, the mass CE of glucose to both PHB and glycogen of 51 ± 15% (w/w) was obtained, while the mass CE of acetate to these two biopolymers was 71 ± 16% (w/w). These optimized cultures exhibited CE of glucose or acetate to both PHB and glycogen at the higher levels than those of previous reports in photoheterotrophic microbes. This single-stage photoheterotrophic cultivation may be applied for production of biopolymers by other photoheterotrophic microbes. © 2021, The Author(s), under exclusive licence to Springer Nature B.V.},

note = {cited By 0},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Singhon20212833,

title = {Increased bioproduction of glycogen, lipids, and poly(3-hydroxybutyrate) under partial supply of nitrogen and phosphorus by photoautotrophic cyanobacterium Synechocystis sp. PCC 6803},

author = {P. Singhon and O. Phoraksa and A. Incharoensakdi and T. Monshupanee},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85106517480&doi=10.1007%2fs10811-021-02494-0&partnerID=40&md5=2323df1b1c92c8e2baa813cb6eacf0cd},

doi = {10.1007/s10811-021-02494-0},

year  = {2021},

date = {2021-01-01},

journal = {Journal of Applied Phycology},

volume = {33},

number = {5},

pages = {2833-2843},

abstract = {Under an adequate supply of nitrogen (N) and phosphorus (P), the cyanobacterium Synechocystis sp. PCC 6803 produced a high biomass yield but with a low storage level of glycogen and poly(3-hydroxybutyrate) (PHB). In contrast, deprivation of N or P stimulated accumulation of glycogen and PHB, but reduced biomass yield. In addition, deprivation of N or P had no apparent effect on total lipid accumulation. Here, we examined how partial supply of N or P has effects on the biomass yield and production of glycogen, PHB, and lipids. The N or P supply in the culture media was orderly decreased from 100% (set according to the standard BG11 medium) to 50, 35, 20, 10, and 0%. A 100% supply of both N and P produced biomass of up to 1.4 g L−1, while a 35–50% N supply slightly reduced the biomass to 1.2–1.3 g L−1, and no apparent change in the biomass was detected under a 20–50% P supply. A maximum glycogen accumulation of 51.8% (w/w dry weight; DW) was obtained under 20% P supply, while the maximum PHB storage of 6.3% (w/w DW) was obtained with 10% P supply. Lipid accumulation peaked at 12.7% (w/w DW) under 0% P supply. The maximal co-production of these three bioproducts was obtained under 20% P supply at 765 mg L−1 which is the sum of 72 mg L−1 PHB (corresponding to 5.1% w/w DW PHB), 618 mg L−1 glycogen (43.8% w/w DW glycogen), and 75 mg L−1 lipid (5.3% w/w DW lipid) from the total biomass production of 1.4 g L−1. The glycogen production (618 mg L−1) was higher than those previously reported for Synechocystis. The results indicated that a proper adjustment of initial N and P supplies in the culture is crucial for maximal production of these three bioproducts by Synechocystis. © 2021, The Author(s), under exclusive licence to Springer Nature B.V.},

note = {cited By 3},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Chongcharoenchaikul2022,

title = {Synergistic effect of cuttlebone particles and non-rubber components on reinforcing ability of natural rubber and synthetic isoprene rubber composites},

author = {T. Chongcharoenchaikul and K. Miyaji and P. Junkong and S. Poompradub and Y. Ikeda},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85126747347&doi=10.1002%2fapp.52375&partnerID=40&md5=9f4e3d41d00c57d3adb8edb97fc26c16},

doi = {10.1002/app.52375},

year  = {2022},

date = {2022-01-01},

journal = {Journal of Applied Polymer Science},

volume = {139},

number = {24},

abstract = {The comparison of the reinforcing ability of cuttlebone (CTBO) particles in natural and synthetic isoprene rubber (NR and IR, respectively) composites were investigated. The CTBO particles could reinforce in both NR and IR composites. However, the improvement of tensile properties in CTBO-filled NR composites was higher than that of IR composites due to the presence of non-rubber components in NR. The non-rubber components promoted the strong interfacial adhesions with filler and rubbery matrix to increase the bound rubber content. These results affected the increase of the stress softening degree, hysteresis loss, residual strain during cyclic deformation and the volume fraction of constrained rubber region (immobilized area of rubber) in CTBO-filled NR composites. The atomic force microscopy evidently revealed good compatibility between the CTBO particles and the NR matrix due to the presence of organic components from CTBO particles and from non-rubber components in NR. Accordingly, the CTBO particles and non-rubber components play important roles in improving the properties of green rubber composite materials. © 2022 Wiley Periodicals LLC.},

note = {cited By 1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Chongcharoenchaikul202213557,

title = {Effects of organic components in cuttlebone on the morphological and mechanical properties of peroxide cross-linked cuttlebone/natural rubber composites},

author = {T. Chongcharoenchaikul and K. Miyaji and P. Junkong and S. Poompradub and Y. Ikeda},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85131463277&doi=10.1039%2fd2ra01885c&partnerID=40&md5=9c94aa20517d542af340d5e38c517aaf},

doi = {10.1039/d2ra01885c},

year  = {2022},

date = {2022-01-01},

journal = {RSC Advances},

volume = {12},

number = {22},

pages = {13557-13565},

abstract = {The clarification of the role of organic components in cuttlebone particles on the morphological and mechanical properties in terms of the strain-induced crystallization (SIC) of peroxide cross-linked cuttlebone/natural rubber (NR) composites was revealed for the first time in this study. The organic components in cuttlebone particles affected the increased bound rubber layers and the decreased rubber chain orientation due to the formation of interfacial interactions (filler-to-filler and/or filler-to-rubber interactions). During SIC, the presence of organic components in cuttlebone particles did not significantly affect the crystallinity index and crystallite size in cuttlebone/NR composites. The increased moduli in the stress-strain curve resulted from the presence of biofiller, SIC, and organic components in the cuttlebone. Therefore, the presence of organic components in biofiller is an important factor in improving the mechanical properties of green rubber composite materials. © 2022 The Royal Society of Chemistry.},

note = {cited By 0},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Tumnantong2022,

title = {Preparation of poly(methyl methacrylate)-silica nanocomposites via DMP-assisted RAFT polymerization and NR/PMMA-RAFT-SiO2 hybrid membrane for pervaporation},

author = {D. Tumnantong and K. Srisamrid and S. Poompradub and P. Prasassarakich},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85125237239&doi=10.1016%2fj.eurpolymj.2022.111088&partnerID=40&md5=79a492272199bf4b80c53b975af5205e},

doi = {10.1016/j.eurpolymj.2022.111088},

year  = {2022},

date = {2022-01-01},

journal = {European Polymer Journal},

volume = {168},

abstract = {Poly(methyl methacrylate)-silica (PMMA-RAFT-SiO2) nanocomposite emulsion was successfully synthesized via a combination of differential microemulsion polymerization (DMP) and reversible addition-fragmentation chain-transfer (RAFT) polymerization. The PMMA-RAFT-SiO2 emulsion with a low SiO2 loading (10 wt%) exhibited nano-sized particles (49 nm) with a core–shell structure and a high monomer conversion (88%). Natural rubber (NR) latex filled with PMMA-RAFT-SiO2 nanocomposites was cast to form hybrid membranes for pervaporation. The addition of SiO2 enhanced the physical properties of the composite membranes. The NR/PMMA-RAFT-SiO2 hybrid membrane with 4.0 wt% SiO2 content showed good mechanical properties. The hybrid membranes had hydrophilic properties and gave a high separation performance in the pervaporation of an ethanol solution (flux = 1,873–2,245 g/m2hr and pure water in permeate = 98–99%). Thus, the PMMA-RAFT-SiO2 nanocomposites had a great potential to be used as an effective filler in hybrid membranes for separation technology. © 2022 Elsevier Ltd},

note = {cited By 1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Tumnantong2021899,

title = {Carbon dioxide adsorption of diallylamine-modified natural rubber with modified silica particles},

author = {D. Tumnantong and K. Panploo and B. Chalermsinsuwan and P. Prasassarakich and S. Poompradub},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85115332381&doi=10.3144%2fexpresspolymlett.2021.72&partnerID=40&md5=f808580bc9d22b2068c79cdee351bc14},

doi = {10.3144/expresspolymlett.2021.72},

year  = {2021},

date = {2021-01-01},

journal = {Express Polymer Letters},

volume = {15},

number = {9},

pages = {899-909},

abstract = {In this study, modified natural rubber (MNR) was used as a solid adsorbent for carbon dioxide (CO2) capture. The chemical structure of the NR latex was modified by diallylamine. Moreover, the silica particles were modified by (3-aminopropyl)trimethoxysilane, N-[(3-trimethoxysilyl)propyl]ethylenediamine, or N-[(3-trimethoxysilyl)propyl]diethyl-enetriamine (mono-, di-and tri-amines) to improve the CO2 capture ability of the MNR. The CO2 adsorption capacity of the MNR foam composite was increased 3-to 5-fold after filling with unmodified or modified silica particles. The mechanism for CO2 adsorption of the MNR composite was a combination of physisorption and chemisorption. At 100 °C, the highest CO2 adsorption capacity of MNR foam composite (10.35 mg/g of adsorbent) was obtained by adding tri-amine-modified silica particles. Finally, the MNR foam composite material could be regenerated process for more than 20 CO2 adsorption cycles. © BME-PT.},

note = {cited By 1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Panploo2020,

title = {Effect of amine types and temperature of a natural rubber based composite material on the carbon dioxide capture},

author = {K. Panploo and B. Chalermsinsuwan and S. Poompradub},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85087907671&doi=10.1016%2fj.cej.2020.125332&partnerID=40&md5=8254fc1e0564505b09a978c2bdae3c6c},

doi = {10.1016/j.cej.2020.125332},

year  = {2020},

date = {2020-01-01},

journal = {Chemical Engineering Journal},

volume = {402},

abstract = {Solid natural rubber (NR) was chosen to prepare a carbon dioxide (CO2) adsorbent material in order to solve the disadvantage of using NR latex as the adsorbent. The effect of modifier type on the CO2 adsorption capacity of the NR/silica foam composites was investigated under ambient temperature and pressure. The CO2 adsorption capacity of the NR/silica foam composite material increased from 1.81 to 6.71 mg/g after filling with silica particles modified by 3-aminopropyltrimethoxysilane. The NR/silica foam composite material filled with silica particles modified with a short chain structure (3-aminopropyltrimethoxysilane) showed a higher CO2 adsorption capacity than those with a longer modifying chain structure (N-[(3-trimethoxysilyl)propyl]ethylenediamine or N-[(3-trimethoxysilyl)propyl]diethylenetriamine). The increasing of adsorption temperature caused the chains of the long-chain modifier on the silica particle surface to become more flexible, resulting in a high selectivity for capturing CO2. The NR/silica foam composite filled with unmodified or modified silica particles showed a high reusability compared to NR foam. © 2020 Elsevier B.V.},

note = {cited By 4},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Boontip2021,

title = {Strategies for Poly(3-hydroxybutyrate) Production Using a Cold-Shock Promoter in Escherichia coli},

author = {T. Boontip and R. Waditee-Sirisattha and K. Honda and S. C. Napathorn},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85108182530&doi=10.3389%2ffbioe.2021.666036&partnerID=40&md5=d48ab3126a6f7a874f5e30d4e8817072},

doi = {10.3389/fbioe.2021.666036},

year  = {2021},

date = {2021-01-01},

journal = {Frontiers in Bioengineering and Biotechnology},

volume = {9},

abstract = {The present study attempted to increase poly(3-hydroxybutyrate) (PHB) production by improving expression of PHB biosynthesis operon derived from Cupriavidus necator strain A-04 using various types of promoters. The intact PHB biosynthesis operon of C. necator A-04, an alkaline tolerant strain isolated in Thailand with a high degree of 16S rRNA sequence similarity with C. necator H16, was subcloned into pGEX-6P-1, pColdI, pColdTF, pBAD/Thio-TOPO, and pUC19 (native promoter) and transformed into Escherichia coli JM109. While the phaCA–04 gene was insoluble in most expression systems tested, it became soluble when it was expressed as a fusion protein with trigger factor (TF), a ribosome associated bacterial chaperone, under the control of a cold shock promoter. Careful optimization indicates that the cold-shock cspA promoter enhanced phaCA–04 protein expression and the chaperone function of TF play critical roles in increasing soluble phaCA–04 protein. Induction strategies and parameters in flask experiments were optimized to obtain high expression of soluble PhaCA–04 protein with high YP/S and PHB productivity. Soluble phaCA–04 was purified through immobilized metal affinity chromatography (IMAC). The results demonstrated that the soluble phaCA–04 from pColdTF-phaCABA–04 was expressed at a level of as high as 47.4 ± 2.4% of total protein and pColdTF-phaCABA–04 enhanced soluble protein formation to approximately 3.09−4.1 times higher than that from pColdI-phaCABA–04 by both conventional method and short induction method developed in this study. Cultivation in a 5-L fermenter led to PHB production of 89.8 ± 2.3% PHB content, a YP/S value of 0.38 g PHB/g glucose and a productivity of 0.43 g PHB/(L.h) using pColdTF-phaCABA–04. The PHB film exhibited high optical transparency and possessed Mw 5.79 × 105 Da, Mn 1.86 × 105 Da, and PDI 3.11 with normal melting temperature and mechanical properties. © Copyright © 2021 Boontip, Waditee-Sirisattha, Honda and Napathorn.},

note = {cited By 2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Napathorn2021,

title = {Polyhydroxybutyrate (PHB) Production Using an Arabinose-Inducible Expression System in Comparison With Cold Shock Inducible Expression System in Escherichia coli},

author = {S. C. Napathorn and S. Visetkoop and O. Pinyakong and K. Okano and K. Honda},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85106005665&doi=10.3389%2ffbioe.2021.661096&partnerID=40&md5=1f10e7aabb396bf8809dfda97d897abf},

doi = {10.3389/fbioe.2021.661096},

year  = {2021},

date = {2021-01-01},

journal = {Frontiers in Bioengineering and Biotechnology},

volume = {9},

abstract = {Cupriavidus necator strain A-04 has shown 16S rRNA gene identity to the well-known industrial strain C. necator H16. Nevertheless, the cell characteristics and polyhydroxyalkanoate (PHA) production ability of C. necator strain A-04 were different from those of C. necator H16. This study aimed to express PHA biosynthesis genes of C. necator strain A-04 in Escherichia coli via an arabinose-inducible expression system. In this study, the PHA biosynthesis operon of C. necator strain A-04, consisting of three genes encoding acetyl-CoA acetyltransferase (phaAA–04, 1182 bp, 40.6 kDa), acetoacetyl-CoA reductase (phaBA–04, 741 bp, 26.4 kDa) and PHB synthase Class I (phaCA–04, 1770 bp), was identified. Sequence analysis of the phaAA–04, phaBA–04, and phaCA–04 genes revealed that phaCA–04 was 99% similar to phaCH16 from C. necator H16. The difference in amino acid residue situated at position 122 of phaCA–04 was proline, whereas that of C. necator H16 was leucine. The intact phaCABA–04 operon was cloned into the arabinose-inducible araBAD promoter and transformed into E. coli strains Top 10, JM109 and XL-1 blue. The results showed that optimal conditions obtained from shaken flask experiments yielded 6.1 ± 1.1 g/L cell dry mass (CDM), a PHB content of 93.3 ± 0.9% (w/w) and a productivity of 0.24 g/(L⋅h), whereas the wild-type C. necator strain A-04 accumulated 78% (w/w) PHB with a productivity of 0.09 g/(L⋅h). Finally, for the scaled-up studies, fed-batch cultivations by pH-stat control in a 5-L fermenter of E. coli strains XL1-Blue harboring pBAD/Thio-TOPO-phaCABA–04 and pColdTF-phaCABA–04 in MR or LB medium, leading to a PHB production of 31.4 ± 0.9 g/L at 54 h with a PHB content of 83.0 ± 3.8% (w/w), a CDM of 37.8 ± 1.2 g/L, a YP/S value of 0.39 g PHB/g glucose and a productivity of 0.6 g PHB/(L⋅h) using pColdTF-phaCABA–04 in MR medium. In addition, PHB production was 29.0 ± 1.1 g/L with 60.2 ± 2.3% PHB content in the CDM of 53.1 ± 1.0 g/L, a YP/S value of 0.21 g PHB/g glucose and a productivity of 0.4 g PHB/(L⋅h) using pBAD/Thio-TOPO-phaCABA–04 in LB medium. Thus, a relatively high PHB concentration and productivity were achieved, which demonstrated the possibility of industrial production of PHB. © Copyright © 2021 Napathorn, Visetkoop, Pinyakong, Okano and Honda.},

note = {cited By 1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Unrean2021,

title = {Lignin to polyhydroxyalkanoate bioprocessing by novel strain of Pseudomonas monteilii},

author = {P. Unrean and S. C. Napathorn and K. L. Tee and T. S. Wong and V. Champreda},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85104824569&doi=10.1007%2fs13399-021-01525-7&partnerID=40&md5=a64a1e169ec24488b9f7fde69bab36ee},

doi = {10.1007/s13399-021-01525-7},

year  = {2021},

date = {2021-01-01},

journal = {Biomass Conversion and Biorefinery},

abstract = {A fed-batch process using lignin-rich black liquor, derived from alkaline pretreated sugarcane bagasse, as carbon source was optimized for the production of polyhydroxyalkanoate (PHA) by Pseudomonas monteilii. Different C:N ratio and pulse feeding schemes in batch and fed-batch processes were studied for their effects on lignin conversion efficiency to cell growth and PHA synthesis. Under optimized feeding profiles and overall C:N ratio of 0.18 g lignin:mg NH4Cl, 238 mg/L of PHA equivalent to 13% PHA content of the cell dry weight was achieved. This was enhanced by 1.9 folds compared to batch process under similar C:N ratio. The produced biopolymer composed of two dominant monomers, 3-hydroxybutyrate and 3-hydroxydecanoate, based on GC-MS analysis. Overall, this work demonstrates the effect of fed-batch fermentation parameters for the synthesis of PHA from waste black liquor, which could serve as a base to lead further investigation in developing integrated lignin bioprocessing platform for cost-effective sustainable biorefineries in Bio-Circular-Green economy. Graphical abstract: [Figure not available: see fulltext.] © 2021, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.},

note = {cited By 2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Khomlaem2020,

title = {Defatted Chlorella biomass as a renewable carbon source for polyhydroxyalkanoates and carotenoids co-production},

author = {C. Khomlaem and H. Aloui and A. R. Deshmukh and J. -H. Yun and H. -S. Kim and S. C. Napathorn and B. S. Kim},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85090425170&doi=10.1016%2fj.algal.2020.102068&partnerID=40&md5=762e04b063281bf7dc39f2b98c61e852},

doi = {10.1016/j.algal.2020.102068},

year  = {2020},

date = {2020-01-01},

journal = {Algal Research},

volume = {51},

abstract = {Defatted Chlorella biomass was investigated as an unconventional and promising carbon source for the co-production of polyhydroxyalkanoates and cartenoids via batch cultivation of Paracoccus sp. LL1. 10% (w/v) of algal biomass was subjected to acid hydrolysis using different concentrations (0.1, 0.2, 0.3, and 0.5 N) of either sulfuric or hydrochloric acid to yield fermentable sugars. Regardless of the acid type, reducing sugar content significantly increased with an increasing acid concentration, reaching a maximum yield of 52 and 46 g/L respectively for hydrochloric and sulfuric acid catalyzed hydrolysis at the highest acid concentrations (p < 0.05). Shake flask fermentation process showed the ability of Paracoccus sp. LL1 to convert fermentable sugars from 0.3 N hydrochloric acid catalyzed hydrolysis into polyhydroxyalkanoates and carotenoids with a maximum product concentration of 1.48 g/L (i.e., 37.4% of dry cell weight) and 6.08 mg/L, respectively. Moreover, 144 and 92% increases in polyhydroxyalkanoates and carotenoids production (i.e., 3.62 g/L polyhydroxyalkanoates and 11.7 mg/L carotenoids) was recorded upon upscaling the biotransformation of defatted Chlorella biomass hydrolysate in 5 L fermenter by Paracoccus sp. LL1. Notably, these results corresponded to 92 and 54% greater production of polyhydroxyalkanoates and carotenoids than those observed in fermenter operated under identical conditions with glucose as a carbon source. Subsequent analyses of gas chromatography, Fourier transform infrared spectroscopy, and 1H nuclear magnetic resonance spectroscopy further identified the accumulated polyhydroxyalkanoate as a copolymer of 3-hydroxybutyrate and 3-hydroxyvalerate with a 3-hydroxyvalerate fraction of 6.22 mol%. © 2020 Elsevier B.V.},

note = {cited By 14},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Punrat202011,

title = {Production of a sophorolipid biosurfactant by Wickerhamomyces anomalus MUE24 and its use for modification of rice flour properties},

author = {T. Punrat and J. Thaniyavarn and S. C. Napathorn and J. Anuntagool and S. Thaniyavarn},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85107506018&doi=10.2306%2fSCIENCEASIA1513-1874.2020.013&partnerID=40&md5=5ff0c987daaa97393f02b80ae2a3e29f},

doi = {10.2306/SCIENCEASIA1513-1874.2020.013},

year  = {2020},

date = {2020-01-01},

journal = {ScienceAsia},

volume = {46},

number = {1},

pages = {11-18},

abstract = {A sophorolipid biosurfactant from Wickerhamomyces anomalus MUE24 was produced upon cultivating in a medium containing soybean oil and glucose with an initial pH of 4.5 at 30 °C in shake flask at 200 rpm. After 7 days of cultivation, cells released surfactant into culture medium at 0.55 g/l. The biosurfactant obtained was able to reduce surface tension of the medium from 52.5 mN/m to 36.0 mN/m. A scale up in batch cultivation was further performed in a 5-liter fermenter controlled at 30 °C 1 vvm, initial pH 4.5. After 72 h of cultivation, biosurfactant concentration was 19.41 g/l. An extraction from whole cell-containing culture could recover the biosurfactant at 34.06 g/l with a critical micelle concentration of 116 mg/l. Further characterization showed that the crude extract of biosurfactant was able to emulsify various types of vegetable oils such as canola oil, sunflower oil and soybean oil. Addition of the crude extract of biosurfactant into rice flour could improve retrogradation, water-holding capacity and swelling power of rice flour. © 2020 Science Society of Thailand under Royal Patronage. All rights reserved.},

note = {cited By 2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Kheeree2022,

title = {Discovery of calcium-binding peptides derived from defatted lemon basil seeds with enhanced calcium uptake in human intestinal epithelial cells, Caco-2},

author = {N. Kheeree and K. Kuptawach and S. Puthong and P. Sangtanoo and P. Srimongkol and P. Boonserm and O. Reamtong and K. Choowongkomon and A. Karnchanatat},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85126697214&doi=10.1038%2fs41598-022-08380-0&partnerID=40&md5=c52d43500ace67272fcf4fa0fe7272cc},

doi = {10.1038/s41598-022-08380-0},

year  = {2022},

date = {2022-01-01},

journal = {Scientific Reports},

volume = {12},

number = {1},

abstract = {It is anticipated that calcium-chelating peptides may serve to enhance the absorption of calcium. This research examined defatted lemon basil seeds (DLBS) which had been treated with Alcalase under optimized parameters for the degree of hydrolysis for proteolysis, discovering that the activity for calcium-binding in a competitive condition with phosphate ion was 60.39 ± 1.545%. The purification of the hydrolysates was performed via ultrafiltration along with reversed-phase high performance liquid chromatography (RP-HPLC). Determination of the purified peptide amino acid sequence was confirmed for both peptides and reported as Ala-Phe-Asn-Arg-Ala-Lys-Ser-Lys-Ala-Leu-Asn-Glu-Asn (AFNRAKSKALNEN; Basil-1), and Tyr-Asp-Ser-Ser-Gly-Gly-Pro-Thr-Pro-Trp-Leu-Ser-Pro-Tyr (YDSSGGPTPWLSPY; Basil-2). The respective activities for calcium-binding were 38.62 ± 1.33%, and 42.19 ± 2.27%. Fluorescence spectroscopy, and fourier transform infrared spectroscopy were employed in order to assess the chelating mechanism between calcium and the peptides. It was found that the calcium ions took place through the activity of the amino nitrogen atoms and the oxygen atoms on the carboxyl group. Moreover, both of these peptides served to improve calcium transport and absorption in Caco-2 cell monolayers, depending on the concentration involved. It was revealed that the peptide-calcium complexes offered an increased calcium absorption percentage when compared to free calcium at similar concentrations. It might be concluded that the peptide within the peptide-calcium complex can promote calcium absorption through both active and passive transport pathways by increasing calcium concentration and promoting cell membrane interaction. Accordingly, DLBS protein can be considered a strong potential source of protein which can be used to produce calcium-binding peptides and might therefore play a role in the production of nutraceutical foods as a bioactive ingredient. © 2022, The Author(s).},

note = {cited By 3},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Srimongkol2022,

title = {Microalgae-based wastewater treatment for developing economic and environmental sustainability: Current status and future prospects},

author = {P. Srimongkol and P. Sangtanoo and P. Songserm and W. Watsuntorn and A. Karnchanatat},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85138332033&doi=10.3389%2ffbioe.2022.904046&partnerID=40&md5=a9088b2881cf34a9b85060ae80271f5e},

doi = {10.3389/fbioe.2022.904046},

year  = {2022},

date = {2022-01-01},

journal = {Frontiers in Bioengineering and Biotechnology},

volume = {10},

abstract = {Over the last several decades, concerns about climate change and pollution due to human activity has gained widespread attention. Microalgae have been proposed as a suitable biological platform to reduce carbon dioxide, a major greenhouse gas, while also creating commercial sources of high-value compounds such as medicines, cosmetics, food, feed, and biofuel. Industrialization of microalgae culture and valorization is still limited by significant challenges in scaling up the production processes due to economic constraints and productivity capacities. Therefore, a boost in resource usage efficiency is required. This enhancement not only lowers manufacturing costs but also enhancing the long-term viability of microalgae-based products. Using wastewater as a nutrient source is a great way to reduce manufacturing costs. Furthermore, water scarcity is one of the most important global challenges. In recent decades, industrialization, globalization, and population growth have all impacted freshwater resources. Moreover, high amounts of organic and inorganic toxins in the water due to the disposal of waste into rivers can have severe impacts on human and animal health. Microalgae cultures are a sustainable solution to tertiary and quaternary treatments since they have the ability to digest complex contaminants. This review presents biorefineries based on microalgae from all angles, including the potential for environmental pollution remediation as well as applications for bioenergy and value-added biomolecule production. An overview of current information about microalgae-based technology and a discussion of the associated hazards and opportunities for the bioeconomy are highlighted. Copyright © 2022 Srimongkol, Sangtanoo, Songserm, Watsuntorn and Karnchanatat.},

note = {cited By 0},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Wichai20221449,

title = {Cost Reduction of Gray Oyster Mushroom [Pleurotus sajor-caju (Fr.) Singer] Production Using Lemon Basil (Ocimum citriodorum Vis.) Straw as a Substrate},

author = {T. Wichai and S. Sooksai and S. Noitang and W. Sukaead and P. Chanprapai and J. Piapukiew and A. Karnchanatat and R. Sawangkeaw},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85117951134&doi=10.1007%2fs12649-021-01617-5&partnerID=40&md5=303f2b684e7cb5a3fdebb5172fb8e1c5},

doi = {10.1007/s12649-021-01617-5},

year  = {2022},

date = {2022-01-01},

journal = {Waste and Biomass Valorization},

volume = {13},

number = {3},

pages = {1449-1458},

abstract = {Lemon basil straw (LBS) is an agricultural waste comprising approximately 1500 tons every year generated from basil seed production in Thailand. This study demonstrated the application of LBS as an alternative substrate for the cultivation of the gray oyster mushrooms [Pleurotus sajor-caju (Fr.) Singer] to reduce productions costs. LBS has a high nitrogen content and essential oil as bioactive compounds. LBS was mixed with rubber sawdust (RS), the traditional substrate of mushroom cultivation in Thailand, to estimate the maximum loading of LBS in cylindrical baglog cultivation. Furthermore, total phenolic compounds and antioxidant activities of the fruit body were measured. The optimal mass ratios of RS-to-LBS in the baglogs were in the range of 80:20–50:50. Results showed that the optimal amount of LBS enhanced mycelium growth and the biological efficiency of cultivated mushrooms. At a 50:50 RS-to-LBS mass ratio, the cost of baglog production was reduced by approximately 25% because the cost of LBS was lower than that of RS. Using LBS as replacement substrate at an optimal RS-to-LBS mass ratio not only reduced mushroom production costs but also promoted mycelium growth, biological efficiency, and antioxidant activity of the fruit body. Graphic Abstract: [Figure not available: see fulltext.] © 2021, The Author(s), under exclusive licence to Springer Nature B.V.},

note = {cited By 3},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Wichai20221449b,

title = {Cost Reduction of Gray Oyster Mushroom [Pleurotus sajor-caju (Fr.) Singer] Production Using Lemon Basil (Ocimum citriodorum Vis.) Straw as a Substrate},

author = {T. Wichai and S. Sooksai and S. Noitang and W. Sukaead and P. Chanprapai and J. Piapukiew and A. Karnchanatat and R. Sawangkeaw},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85117951134&doi=10.1007%2fs12649-021-01617-5&partnerID=40&md5=303f2b684e7cb5a3fdebb5172fb8e1c5},

doi = {10.1007/s12649-021-01617-5},

year  = {2022},

date = {2022-01-01},

journal = {Waste and Biomass Valorization},

volume = {13},

number = {3},

pages = {1449-1458},

abstract = {Lemon basil straw (LBS) is an agricultural waste comprising approximately 1500 tons every year generated from basil seed production in Thailand. This study demonstrated the application of LBS as an alternative substrate for the cultivation of the gray oyster mushrooms [Pleurotus sajor-caju (Fr.) Singer] to reduce productions costs. LBS has a high nitrogen content and essential oil as bioactive compounds. LBS was mixed with rubber sawdust (RS), the traditional substrate of mushroom cultivation in Thailand, to estimate the maximum loading of LBS in cylindrical baglog cultivation. Furthermore, total phenolic compounds and antioxidant activities of the fruit body were measured. The optimal mass ratios of RS-to-LBS in the baglogs were in the range of 80:20–50:50. Results showed that the optimal amount of LBS enhanced mycelium growth and the biological efficiency of cultivated mushrooms. At a 50:50 RS-to-LBS mass ratio, the cost of baglog production was reduced by approximately 25% because the cost of LBS was lower than that of RS. Using LBS as replacement substrate at an optimal RS-to-LBS mass ratio not only reduced mushroom production costs but also promoted mycelium growth, biological efficiency, and antioxidant activity of the fruit body. Graphic Abstract: [Figure not available: see fulltext.] © 2021, The Author(s), under exclusive licence to Springer Nature B.V.},

note = {cited By 3},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Kaewsahnguan2021,

title = {A novel angiotensin i-converting enzyme inhibitory peptide derived from the trypsin hydrolysates of salmon bone proteins},

author = {T. Kaewsahnguan and S. Noitang and P. Sangtanoo and P. Srimongkol and T. Saisavoey and O. Reamtong and K. Choowongkomon and A. Karnchanatat},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85114232002&doi=10.1371%2fjournal.pone.0256595&partnerID=40&md5=39b341d7d148c427e609a0753f8d8392},

doi = {10.1371/journal.pone.0256595},

year  = {2021},

date = {2021-01-01},

journal = {PLoS ONE},

volume = {16},

number = {September},

abstract = {When fish are processed, fish bone becomes a key component of the waste, but to date very few researchers have sought to use fish bone to prepare protein hydrolysates as a means of adding value to the final product. This study, therefore, examines the potential of salmon bone, through an analysis of the benefits of its constituent components, namely fat, moisture, protein, and ash. In particular, the study seeks to optimize the process of enzymatic hydrolysis of salmon bone with trypsin in order to produce angiotensin-I converting enzyme (ACE) inhibitory peptides making use of response surface methodology in combination with central composite design (CCD). Optimum hydrolysis conditions concerning DH (degree of hydrolysis) and ACE-inhibitory activity were initially determined using the response surface model. Having thus determined which of the salmon bone protein hydrolysates (SBPH) offered the greatest level of ACE-inhibitory activity, these SBPH were duly selected to undergo ultrafiltration for further fractionation. It was found that the greatest ACE-inhibitory activity was achieved by the SBPH fraction which had a molecular weight lower than 0.65 kDa. This fraction underwent further purification using RP-HPLC, revealing that the F7 fraction offered the best ACE-inhibitory activity. For ACE inhibition, the ideal peptide in the context of the F7 fraction comprised eight amino acids: Phe-Cys-Leu-Tyr-Glu- Leu-Ala-Arg (FCLYELAR), while analysis of the Lineweaver-Burk plot revealed that the FCLYELAR peptide can serve as an uncompetitive ACE inhibitor. An examination of the molecular docking process showed that the FCLYELAR peptide was primarily able to provide ACE-inhibitory qualities as a consequence of the hydrogen bond interactions taking place between ACE and the peptide. Furthermore, upon isolation form the SBPH, the ACEinhibitory peptide demonstrated ACE-inhibitory capabilities in vitro, underlining its potential for applications in the food and pharmaceutical sectors. © 2021 Kaewsahnguan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.},

note = {cited By 2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Wichai20221449,

title = {Cost Reduction of Gray Oyster Mushroom [Pleurotus sajor-caju (Fr.) Singer] Production Using Lemon Basil (Ocimum citriodorum Vis.) Straw as a Substrate},

author = {T. Wichai and S. Sooksai and S. Noitang and W. Sukaead and P. Chanprapai and J. Piapukiew and A. Karnchanatat and R. Sawangkeaw},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85117951134&doi=10.1007%2fs12649-021-01617-5&partnerID=40&md5=303f2b684e7cb5a3fdebb5172fb8e1c5},

doi = {10.1007/s12649-021-01617-5},

year  = {2022},

date = {2022-01-01},

journal = {Waste and Biomass Valorization},

volume = {13},

number = {3},

pages = {1449-1458},

abstract = {Lemon basil straw (LBS) is an agricultural waste comprising approximately 1500 tons every year generated from basil seed production in Thailand. This study demonstrated the application of LBS as an alternative substrate for the cultivation of the gray oyster mushrooms [Pleurotus sajor-caju (Fr.) Singer] to reduce productions costs. LBS has a high nitrogen content and essential oil as bioactive compounds. LBS was mixed with rubber sawdust (RS), the traditional substrate of mushroom cultivation in Thailand, to estimate the maximum loading of LBS in cylindrical baglog cultivation. Furthermore, total phenolic compounds and antioxidant activities of the fruit body were measured. The optimal mass ratios of RS-to-LBS in the baglogs were in the range of 80:20–50:50. Results showed that the optimal amount of LBS enhanced mycelium growth and the biological efficiency of cultivated mushrooms. At a 50:50 RS-to-LBS mass ratio, the cost of baglog production was reduced by approximately 25% because the cost of LBS was lower than that of RS. Using LBS as replacement substrate at an optimal RS-to-LBS mass ratio not only reduced mushroom production costs but also promoted mycelium growth, biological efficiency, and antioxidant activity of the fruit body. Graphic Abstract: [Figure not available: see fulltext.] © 2021, The Author(s), under exclusive licence to Springer Nature B.V.},

note = {cited By 3},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Wichai20221449b,

title = {Cost Reduction of Gray Oyster Mushroom [Pleurotus sajor-caju (Fr.) Singer] Production Using Lemon Basil (Ocimum citriodorum Vis.) Straw as a Substrate},

author = {T. Wichai and S. Sooksai and S. Noitang and W. Sukaead and P. Chanprapai and J. Piapukiew and A. Karnchanatat and R. Sawangkeaw},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85117951134&doi=10.1007%2fs12649-021-01617-5&partnerID=40&md5=303f2b684e7cb5a3fdebb5172fb8e1c5},

doi = {10.1007/s12649-021-01617-5},

year  = {2022},

date = {2022-01-01},

journal = {Waste and Biomass Valorization},

volume = {13},

number = {3},

pages = {1449-1458},

abstract = {Lemon basil straw (LBS) is an agricultural waste comprising approximately 1500 tons every year generated from basil seed production in Thailand. This study demonstrated the application of LBS as an alternative substrate for the cultivation of the gray oyster mushrooms [Pleurotus sajor-caju (Fr.) Singer] to reduce productions costs. LBS has a high nitrogen content and essential oil as bioactive compounds. LBS was mixed with rubber sawdust (RS), the traditional substrate of mushroom cultivation in Thailand, to estimate the maximum loading of LBS in cylindrical baglog cultivation. Furthermore, total phenolic compounds and antioxidant activities of the fruit body were measured. The optimal mass ratios of RS-to-LBS in the baglogs were in the range of 80:20–50:50. Results showed that the optimal amount of LBS enhanced mycelium growth and the biological efficiency of cultivated mushrooms. At a 50:50 RS-to-LBS mass ratio, the cost of baglog production was reduced by approximately 25% because the cost of LBS was lower than that of RS. Using LBS as replacement substrate at an optimal RS-to-LBS mass ratio not only reduced mushroom production costs but also promoted mycelium growth, biological efficiency, and antioxidant activity of the fruit body. Graphic Abstract: [Figure not available: see fulltext.] © 2021, The Author(s), under exclusive licence to Springer Nature B.V.},

note = {cited By 3},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Satjarak20211255,

title = {Hercide atrazine alters the microbiota of the filamentous green alga Cladophora sp. Cultured from Thailand [Herbisid atrazin mengubah mikrobiota alga hijau berfilamen Cladophora sp. yang Dikultur Dari Thailand]},

author = {A. Satjarak and J. Piapukiew and W. Chanthapatchot and K. Ruen-Pham and A. S. Vangnai},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85108577504&doi=10.17576%2fjsm-2021-5005-06&partnerID=40&md5=05879d0bf023a2c8c4ad422cc1a840d1},

doi = {10.17576/jsm-2021-5005-06},

year  = {2021},

date = {2021-01-01},

journal = {Sains Malaysiana},

volume = {50},

number = {5},

pages = {1255-1265},

abstract = {The attached green alga Cladophora known to harbor microbiota that play important roles in ecosystem, is one of the most common freshwater filamentous green algae in rivers globally, including those in the northern part of Thailand. These rivers mostly run through agricultural regions where herbicides are heavily used to improve crop quality and quantity. The extensively-used herbicide atrazine persists in soil sediments through transport by surface runoff to rivers. The effect of such herbicide contamination on Cladophora microbiota in Thailand have not been investigated. To acquire this information, 16S rDNA amplicons were used to compare microbiota of Cladophora sp. cultures treated with a spectrum of atrazine concentrations. The results showed that the Cladophora microbiome included at least 106 possible Operational taxonomic units (OTUs) representing twelve bacterial phyla which are Acidobacteria, Actinobacteria, Armatimonadetes, Chloroflexi, Cyanobacteria, Deinococcus-Thermus, Epsilonbacteraeota, Nitrospirae, Patescibacteria, Planctomycetes, Proteobacteria, and WPS-2, representing both core and local algal bacteria. The presence of atrazine was also correlated with changes in richness of bacterial taxa suggesting that these algal epibiotic bacteria were differently affected by atrazine treatments. © 2021 Penerbit Universiti Kebangsaan Malaysia. All rights reserved.},

note = {cited By 1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Blatrix2021305,

title = {The symbiosis between Philidris ants and the ant-plant Dischidia major includes fungal and algal associates},

author = {R. Blatrix and A. Kidyoo and M. Kidyoo and J. Piapukiew and A. Satjarak and C. Paliyavuth and W. Boonchai and D. McKey},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85100496981&doi=10.1007%2fs13199-021-00751-x&partnerID=40&md5=9f211c2e4e98c0e049925f2b0ab3d51e},

doi = {10.1007/s13199-021-00751-x},

year  = {2021},

date = {2021-01-01},

journal = {Symbiosis},

volume = {83},

number = {3},

pages = {305-315},

abstract = {Dischidia major is an epiphyte with pitcher leaves that serve as nests for ants. We investigated this ant-plant symbiosis in two sites in southeastern Thailand, Rayong and Trat, using a morphological and molecular approach. In our study sites, D. major was colonized by one monomorphic ant species of genus Philidris. The inner surface of the pitcher leaves had a black and green lining composed of intermingled coccoid cells and filaments of algae and fungi, reminiscent of a biofilm structure. Microscopic investigation of the algae suggested they belonged to Trebouxia (coccoid cells) and Trentepohliaceae (filaments). Molecular investigation of environmental samples and pure cultures of the fungi revealed five species of Chaetothyriales and four species of Capnodiales, among which two have already been isolated from ant-plant symbioses in Africa and South America and five were described species known from various environments around world. One appears to be an undescribed species. Thus, most fungal associates were likely ubiquitous species. Our study highlights the need to include the identity and functional ecology of microbes in studies of the evolutionary and functional ecology of ant-plant symbioses. © 2021, The Author(s), under exclusive licence to Springer Nature B.V. part of Springer Nature.},

note = {cited By 0},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Pachit2020,

title = {Temporal changes in enzyme activities associated with ectomycorrhizas and soil from secondary deciduous dipterocarp forest fragments},

author = {P. Pachit and N. R. Disyatat and J. Piapukiew},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85087313975&doi=10.1016%2fj.pedobi.2020.150661&partnerID=40&md5=53f99a74d3c00ad4727c305867488236},

doi = {10.1016/j.pedobi.2020.150661},

year  = {2020},

date = {2020-01-01},

journal = {Pedobiologia},

volume = {81-82},

abstract = {Ectomycorrhizal (ECM) fungi form a symbiotic association with host trees, functionally promoting the nutrient uptake in the hosts via their secretion of enzymes to degrade organic compounds. Because of the involvement in nutrient cycling, ECM fungi are important to the establishment of host trees and forest recovery. In Thailand, ECM fungi are mostly associated with host tree species in the Dipterocarpaceae found in deciduous dipterocarp forests (DDFs), some of which are secondary forests naturally recovering after abandonment of logging and agriculture. To determine the function of ECM fungi in secondary DDFs, temporal changes in the ECM community structure and potential activities of three enzymes involved in nitrogen, carbon, and phosphorus cycling (β-N-acetylglucosaminidase, β-glucosidase, and acid phosphatase) from ECM roots and soils were investigated in two secondary DDF fragments in the Saraburi and Nan provinces of Thailand. A total of 180 soil samples from each forest were collected at four times across the wet and dry seasons from June 2015 to May 2016. Soil moisture content, total nitrogen, organic matter, and available phosphorus contents were measured. The results showed temporal changes in the ECM fungal communities and relative enzyme activities throughout the study period, although differences between the wet and dry seasons were not detected. The enzyme profiles associated with ECM roots were significantly correlated to the ECM communities in both DDFs. Moreover, the total nitrogen and soil moisture content influenced the temporal variation of ECM and soil enzyme profiles. These findings suggest the effect of edaphic factors combined with the ECM communities on their enzyme activities in secondary DDFs in Thailand. © 2020 Elsevier GmbH},

note = {cited By 0},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Piyapattanamongkol2022,

title = {Physicochemical properties and viscoelastic behavior of rice–mung bean composite flour systems as potential ingredients for plant-based foods},

author = {T. Piyapattanamongkol and V. Kongpensook and K. Tananuwong},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85135135656&doi=10.1002%2fcche.10588&partnerID=40&md5=962d86bc0b23c617726b2ca453bd81dc},

doi = {10.1002/cche.10588},

year  = {2022},

date = {2022-01-01},

journal = {Cereal Chemistry},

abstract = {Background and Objectives: Composite flour (CF) from cereals and legumes has been used to formulate plant-based and/or gluten-free food products. Fundamental knowledge regarding the properties of CF is required to formulate food products with desirable qualities. This study aimed to investigate the pasting properties, thermal properties, and viscoelastic behavior of CF, containing rice flour (RF), mung bean flour (MF), and mung bean protein concentrate (MPC). The protein contents of the CF were 17.6%-23.6% on a wet basis. Findings: The CF containing the highest proportion of RF had the highest pasting temperature, peak viscosity, breakdown, setback, and melting enthalpy of retrograded amylopectin. The deconvoluted thermogram from differential scanning calorimetry (DSC) clearly distinguished the endotherms that arose from starch gelatinization, protein denaturation, and melting of the amylose−lipid complex. The gelatinization endotherm of CF was mainly governed by RF. CF containing a higher proportion of RF and MPC had stronger elastic behavior. Conclusions: The type and composition of the flour greatly affected the physicochemical and viscoelastic properties of the CF. RF played an important role in the pasting and thermal properties of the CF samples. Combining RF and MPC could be used to enhance the elastic behavior of dough from CF systems. Significance and Novelty: Deconvoluted DSC peaks provided insight into the multiple thermal transitions that occurred during the processing of CF-based foods. The overall results from this study could help in the formulation of food containing rice−mung bean CF in excess water or in a dough system. © 2022 Cereals & Grains Association.},

note = {cited By 0},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Jiamjariyatam2016445,

title = {Prediction Models for Textural Properties of Puffed Rice Starch Product by Relative Crystallinity},

author = {R. Jiamjariyatam and V. Kongpensook and P. Pradipasena},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84989838518&doi=10.1111%2fjfq.12218&partnerID=40&md5=a2e3ec635acbcfedf1d6e6cf1b4d9297},

doi = {10.1111/jfq.12218},

year  = {2016},

date = {2016-01-01},

journal = {Journal of Food Quality},

volume = {39},

number = {5},

pages = {445-455},

abstract = {This research aimed to determine the textural attributes of fried puffed rice starch products by the relative crystallinity (RC) of pellets (RCp). Three factors, amylose content (AC), aging time and cooling rate were varied to obtain various levels of RCp. The different textural characteristics of the puffed rice products were evaluated by instrumental and descriptive sensory analysis. The higher AC and longer aging time resulted in higher RCp. Pellets with higher RCp had smaller and denser air cell structures and lower expansion, resulting in higher crispness, hardness and fracturability. High correlation between the RCp and the instrumental and the sensory texture characteristics were determined and regression models (with R2 > 0.80) were established. These models can be used to predict some properties of puffed products, such as crispness, hardness, and brittleness. Panelists rated samples with an AC of 9% (in a range of 2.8–3.7% RCp) highest in appearance and texture scores. Practical Applications: The RCp and textural properties of puffed products can be affected by many factors including ingredients and process parameters. This study provides a better understanding of both the textural characteristics of puffed rice starch products using sensory and instrumental approaches, but also their correlations. The relationship between the RCp and sensory evaluation data can be applied for the prediction of puffing quality. © 2016 Wiley Periodicals, Inc.},

note = {cited By 1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Janjarasskul2016166,

title = {Shelf life extension of sponge cake by active packaging as an alternative to direct addition of chemical preservatives},

author = {T. Janjarasskul and K. Tananuwong and V. Kongpensook and S. Tantratian and S. Kokpol},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84967194545&doi=10.1016%2fj.lwt.2016.04.049&partnerID=40&md5=c4dba3893c98115c3260ee18e6e20228},

doi = {10.1016/j.lwt.2016.04.049},

year  = {2016},

date = {2016-01-01},

journal = {LWT},

volume = {72},

pages = {166-174},

abstract = {To extend the shelf life of sponge cake by an O2 scavenger (OS) and an ethanol emitter (EE) as alternatives to chemical preservatives, sponge cake samples were packaged with an active packaging system and a high barrier pouch, polyvinylidene chloride (PVDC)-coated nylon or nylon. Effects studied included the initial rate of O2 reduction, total O2-scavenging capacity to maintain critically low O2 concentration through storage, and effects of ethanol on microbial growth and evolution of headspace CO2 and O2. Active packaging system effectively extended mold-free shelf life of preservative-free cake from ~1 day (control) to more than 42 days. By significantly retarding microbial growth and activities, active packaging shifts the critical shelf life determination reaction to physicochemical deteriorations. OS retarded lipid oxidation and enhanced color stability of the cake. EE delayed staling. PVDC-coated nylon enhanced the efficiency of active packaging better than nylon. The combination of OS and EE synergistically delayed microbial spoilage as well as physical and chemical deteriorations. Application of active packaging systems is an effective alternative preservation technology for prolonging the shelf life of highly perishable sponge cake without the direct addition of chemical preservatives into the bakery formula. © 2016.},

note = {cited By 31},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Jiamjariyatam201516,

title = {Effects of amylose content, cooling rate and aging time on properties and characteristics of rice starch gels and puffed products},

author = {R. Jiamjariyatam and V. Kongpensook and P. Pradipasena},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84922496679&doi=10.1016%2fj.jcs.2014.10.001&partnerID=40&md5=73196cd8c817c0ce6c9b94f40e06fca5},

doi = {10.1016/j.jcs.2014.10.001},

year  = {2015},

date = {2015-01-01},

journal = {Journal of Cereal Science},

volume = {61},

pages = {16-25},

abstract = {This study aimed at investigating the effects of amylose content (AC) of 0.12-19.00% w/w on dry basis, cooling rate (1, 3, 5, and 9°C/min), and aging time (24, 48, and 72h) on structure, physical properties and sensory attributes of rice starch-based puffed products. They had an influence upon the crystalline type, and the relative crystallinity (RC). The thermal and physical properties of starch gels were also determined. Amorphism was found for starch gels with 0.12% AC. The polymorphisms (B and V) and differential scanning calorimetric endotherms were found for those with AC ≥4.00%. The RC, retrogradation enthalpy (δHr) and gel hardness increased with AC and aging time. The cooling rate did not affect RC, but increased δHr and gel hardness. The higher AC and aging time resulted in higher hardness, fracturability, crispiness and bulk density, but lower expansion ratio and less oiliness of the puffed products. The hardness, fracturability, crispiness and bulk density of puffed products were well correlated with the RC of starch gel. © 2014 Elsevier Ltd.},

note = {cited By 36},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Rumruaytum2014143,

title = {Effect of drying involving fluidisation in superheated steam on physicochemical and antioxidant properties of Thai native rice cultivars},

author = {P. Rumruaytum and C. Borompichaichartkul and V. Kongpensook},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84887076051&doi=10.1016%2fj.jfoodeng.2013.08.025&partnerID=40&md5=a713349df2d71b9cef3fab9b06ed10c4},

doi = {10.1016/j.jfoodeng.2013.08.025},

year  = {2014},

date = {2014-01-01},

journal = {Journal of Food Engineering},

volume = {123},

pages = {143-147},

abstract = {This paper is aiming at investigating the effects of drying process on physicochemical and antioxidant properties of Thai native rice cultivars Sung Yod Phattalung and Nauykaur. A combination of fluidisation and superheated steam is the selected protocol for the first stage of drying. Two-stage drying process, i.e. fluidisation drying under superheated steam as a medium followed by shade drying at ambient temperature is employed to dry wet Thai native paddy at 170 C for different length of time (2.5-4 min), then followed by shade drying at ambient temperature until the final moisture content of the paddy comes down to 13-14% (w.b.). The results of physicochemical and antioxidant properties of native paddy after drying showed that the longer drying time in superheated steam resulted in changes of pasting properties such as peak viscosity, breakdown, final viscosity and set back of the native rice. The values of the pasting properties decreased while pasting temperature increased. The antioxidant activity of Sung Yod Phattalung was reduced, but that of Nauykaur increased when drying time in superheated steam increased. © 2013 Elsevier Ltd. All rights reserved.},

note = {cited By 29},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Techa2023,

title = {Isolation and functional identification of secretin family G-protein coupled receptor from Y-organ of the mud crab, Scylla olivacea},

author = {S. Techa and W. Thongda and P. Bunphimpapha and W. Ittarat and M. Boonbangyang and A. Wilantho and C. Ngamphiw and B. Pratoomchat and P. Nounurai and S. Piyapattanakorn},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85138548333&doi=10.1016%2fj.gene.2022.146900&partnerID=40&md5=06d9f1f9862434067d18861c4dfb9fdf},

doi = {10.1016/j.gene.2022.146900},

year  = {2023},

date = {2023-01-01},

journal = {Gene},

volume = {848},

abstract = {Recently, genes in the superfamily of GPCR are gaining more interest in crustaceans as more evidence shows that they are involved in molting. This study identified four forms of the secretin family of G-protein coupled receptor (GPCR) from the Y-organ of mud crab, Scylla olivacea (ScoGPCR). A full-length sequence of ScoGPCR-B2 was isolated and identified as a lipoprotein receptor while three forms of GPCR in Methuselah-like (Mthl) or B3 subfamilies were reported as ScoGPCR-B3a, -B3b, and -B3c. These four forms exhibit common features of the 7-trans membrane (7TM) domain and distinct aspects in the extracellular region (ECR) at the N-terminus. At the ECR, disulfide bridges are predicted to generate structural stability in all four forms while the putative ScoGPCR-B3 proteins retain conserved Tyr, Trp, Pro, and Phe residues, possibly to form the aromatic-proline interactions and function as key residues for receptor recognition. Expression levels of ScoGPCR-B2 and -B3 in eyestalk, thoracic ganglion, and hindgut between intermolt and premolt stages are similar. Only ScoGPCR-B2 and ScoGPCR-B3a in Y-organ (YO) seem to be premolt-specific responses. An upregulation of ScoGPCR-B2 in YO at the premolt stage is correlated with the demand for cholesterol used in ecdysteroid synthesis, resulting in increased ecdysteroid titers. The effects of ecdysone on YO were pursued by in vitro incubation and revealed that ScoGPCR-B3a and -B3b expressions were induced in a different time frame: early in ScoGPCR-B3b and late in ScoGPCR-B3a. The early response of ScoGPCR-B3b was followed through immunohistology and showed that the newly synthesized protein was located primarily in the cytosol. © 2022 Elsevier B.V.},

note = {cited By 0},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Budi2022583,

title = {Mitogenomes provide insight into complex evolutionary history of freshwater and coastal Irrawaddy dolphin (Orcaella brevirostris Gray, 1866) in Thailand and Indonesia},

author = {T. Budi and S. Piyapattanakorn and D. Kreb and P. Yuda and S. Ninwat and P. Hardwises and P. Prachamkhai and W. Senanan and S. Thongsukdee and J. Phavaphutanon and W. Klinsawat},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85135595125&doi=10.34044%2fj.anres.2022.56.3.14&partnerID=40&md5=75b2b25a1d19146194610ceb89bf2e6a},

doi = {10.34044/j.anres.2022.56.3.14},

year  = {2022},

date = {2022-01-01},

journal = {Agriculture and Natural Resources},

volume = {56},

number = {3},

pages = {583-596},

abstract = {Importance of the work: Irrawaddy dolphins have experienced demographic decline. The lack of genetic information might hamper management decisions necessary for restoring population viability. Objectives: To apply population genetics and mitogenome phylogenetic analyses to assess genetic diversity and connectivity among Irrawaddy dolphin populations in Indonesia and Thailand. Materials & Methods: Samples from stranded dolphins were collected from Mahakam River and East Kalimantan, Indonesia and from Songkhla Lake and the Lower and Upper Gulf of Thailand. Based on Bayesian time-scaled phylogeny and pairwise population differentiation, the divergence times were estimated between Irrawaddy dolphin lineages and potential historical factors influencing the observed genetic structure were inferred. Results: The Indonesian populations had lower mtDNA diversity compared to Thailand populations. Pairwise population differentiation and analysis of molecular variance based on the control region supported high differentiation among fragmented Thailand and Indonesian populations (FST = 0.6622, p < 0.0001) with 66.21% variation among groups, except for the Songkhla Lake and coastal Lower Gulf populations. Phylogenetic analyses revealed close relationships between some of the Thai and Indonesian haplotypes, suggesting historical freshwater connectivity. There was a lack of monophyly despite the strong support for the Mahakam River clade in Indonesia. The Mahakam River population started to diversify during the late Pleistocene (~304.4 Ka; 95% highest posterior density (HPD) = 157.8–505.3 Ka), possibly due to sea-level fluctuation and changes in palaeodrainage systems. Main finding: The results of the genetic diversity and complex evolutionary history of the Irrawaddy dolphin raise conservation concerns and support management strategies aiming to restore population connectivity and reduce the impacts of genetic and demographic stochasticity. © 2022 Elsevier B.V.. All rights reserved.},

note = {cited By 0},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Burut-Archanai2021671,

title = {Dynamics of Microbial Community During Nitrification Biofilter Acclimation with Low and High Ammonia},

author = {S. Burut-Archanai and D. Ubertino and P. Chumtong and W. Mhuantong and S. Powtongsook and S. Piyapattanakorn},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85112803963&doi=10.1007%2fs10126-021-10056-1&partnerID=40&md5=8c192111627159f4ed1eead34907f2a0},

doi = {10.1007/s10126-021-10056-1},

year  = {2021},

date = {2021-01-01},

journal = {Marine Biotechnology},

volume = {23},

number = {4},

pages = {671-681},

abstract = {The acclimation of a nitrifying biofilter is a crucial and time-consuming task for setting up a recirculating aquaculture system (RAS). Gaining a better understanding of the dynamics of the microbial community during the acclimation period in the system could be useful for the development of mature nitrifying biofilters. In this study, high-throughput DNA sequencing was applied to monitor the microbial communities on a biofilter during the acclimation period (7 weeks) in high (100 mg N/L) and low (5 mg N/L) total ammonia nitrogen (TAN) treatments. Both treatments were successful for developing a mature nitrifying biofilter, dominated by Proteobacteria, Bacteroidetes, and Nitrospirae. Complete nitrification was found after 7 days of biofilter acclimation as indicated by decreasing TAN concentration, increasing nitrate concentration, and high abundances of the nitrifying bacteria, Nitrosomonadaceae and Nitrospiraceae. The beta diversity analysis of microbial communities showed different clustering of the samples between high and low TAN treatment groups. A greater abundance of nitrifying bacteria was found in the high TAN treatments (27–51%) than in the low TAN treatment (15–29%). The bacterial diversity in biofilters acclimated at high TAN concentration (Shannon’s index 5.40–6.15) were lower than those found at low TAN treatment levels (Shannon’s index 6.40–7.01). The higher diversity in biofilters acclimated at low TAN concentrations, consisting of Planctomycetes and Archaea, might benefit the nutrient recycling in the system. Although nitrification activity was observed from the first week of the acclimation period, the acclimation period should be taken as at least 6 weeks for full development of nitrifying biofilm. Moreover, the reduction of potentially pathogenic Vibrio on biofilters was found at that period. © 2021, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.},

note = {cited By 0},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Koyama2021383,

title = {Changes in metabolite concentrations in the abdominal muscle of the kuruma shrimp Marsupenaeus japonicus in response to different salinities},

author = {H. Koyama and H. Kuniyoshi and S. Piyapattanakorn and S. Watabe},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85103876462&doi=10.1007%2fs12562-021-01512-x&partnerID=40&md5=b9381ac5af7417a5673c81144fc873aa},

doi = {10.1007/s12562-021-01512-x},

year  = {2021},

date = {2021-01-01},

journal = {Fisheries Science},

volume = {87},

number = {3},

pages = {383-401},

abstract = {Invertebrates change the metabolite concentrations in their bodies to adapt to environmental salinity. The kuruma shrimp Marsupenaeus japonicus is one such invertebrate. It lives in coastal areas and is also known to change free amino acid concentrations depending on environmental salinity. To examine the relationship between the concentrations of metabolites, including amino acids, in the kuruma shrimp with environmental salinity, metabolome analysis was performed on the abdominal muscle of shrimps acclimated at 17, 34 and 40‰ salinity for 24 h. Principal component analysis revealed that the accumulation patterns of metabolites using the 111 metabolites detected in the shrimps exposed to different salinities were depicted in a salinity-dependent manner. The concentrations of alanine and glutamine were increased following exposure to increasing levels of salinity, suggesting that these free amino acids function in intracellular osmoregulation of the kuruma shrimp. Furthermore, the concentration of glycolytic metabolites was significantly decreased at high salinity. The concentrations of most of the metabolites related to the tricarboxylic acid (TCA) cycle also tended to decrease at high salinity. Considering the levels of expression of the genes related to various metabolic pathways, it seems that glycolysis is accelerated at high salinity. © 2021, Japanese Society of Fisheries Science.},

note = {cited By 0},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Koyama2018,

title = {Changes in free amino acid concentrations and associated gene expression profiles in the abdominal muscle of kuruma shrimp (Marsupenaeus japonicus) acclimated at different salinities},

author = {H. Koyama and N. Mizusawa and M. Hoashi and E. Tan and K. Yasumoto and M. Jimbo and D. Ikeda and T. Yokoyama and S. Asakawa and S. Piyapattanakorn and S. Watabe},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85048339597&doi=10.1242%2fjeb.168997&partnerID=40&md5=63ddf66931de5d6a0e48e3437661ca07},

doi = {10.1242/jeb.168997},

year  = {2018},

date = {2018-01-01},

journal = {Journal of Experimental Biology},

volume = {221},

number = {11},

abstract = {Shrimps inhabiting coastalwaters can survive in a wide range of salinity. However, the molecular mechanisms involved in their acclimation to different environmental salinities have remained largely unknown. In the present study, we acclimated kuruma shrimp (Marsupenaeus japonicus) at 1.7%, 3.4% and 4.0% salinities. After acclimating for 6, 12, 24 and 72 h, we determined free amino acid concentrations in their abdominal muscle, and performed RNA sequencing analysis on this muscle. The concentrations of free amino acids were clearly altered depending on salinity after 24 h of acclimation. Glutamine and alanine concentrations were markedly increased following the increase of salinity. In associationwith such changes,many genes related to amino acid metabolism changed their expression levels. In particular, the increase of the expression level of the gene encoding glutamateammonia ligase, which functions in glutaminemetabolism, appeared to be associated with the increased glutamine concentration at high salinity. Furthermore, the increased alanine concentration at high salinity was likely associated with the decrease in the expression levels of the the gene encoding alanine-glyoxylate transaminase. Thus, there is a possibility that changes in the concentration of free amino acids for osmoregulation in kuruma shrimp are regulated by changes in the expression levels of genes related to amino acid metabolism. © 2018. Published by The Company of Biologists Ltd.},

note = {cited By 8},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Jafari2023,

title = {A decade overview and prospect of spray drying encapsulation of bioactives from fruit products: Characterization, food application and in vitro gastrointestinal digestion},

author = {S. Jafari and S. M. Jafari and M. Ebrahimi and I. Kijpatanasilp and K. Assatarakul},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85136156121&doi=10.1016%2fj.foodhyd.2022.108068&partnerID=40&md5=217f81eb53f338dea4f61a3425bb05c7},

doi = {10.1016/j.foodhyd.2022.108068},

year  = {2023},

date = {2023-01-01},

journal = {Food Hydrocolloids},

volume = {134},

abstract = {Background: The demand for value-added and/or functional food products has skyrocketed in recent years. Fruits and fruit products are of the best sources of bioactive compounds, which provide a variety of health advantages. Spray drying encapsulation is a common and cost-effective method for protecting a variety of bioactive compounds against degradation, controlling or delaying release, and masking unpleasant tastes or odors. Scope and approach: This article summarizes the findings and research (almost 70 studies) on encapsulation by spray drying from fruit products over the last decade. Key findings and conclusions: The composition and type of carriers (wall materials) were important in the encapsulation and maintenance of physicochemical, structural, and functional aspects of microcapsules, according to the findings of a decade of research. Furthermore, the combination of polysaccharide–protein-based wall components as well as the higher wall/core ratio resulted in higher encapsulation efficiency as shown in some studies. Bioactive-microcapsules have been used in food products for enrichment, fortification, coloring, and stability enhancement against oxidation and microbiological proliferation, with promising results. Encapsulation by spray drying was also tested in vitro gastrointestinal settings, however more research is needed to assure low toxicity and improved bioaccessibility. The use of spray drying to microencapsulate bioactive compounds in fruit products could open up new possibilities for the controlled delivery of beneficial compounds to the host, as well as a potentially cost-effective method to prevent and/or treat a wide range of diseases. However, in order to turn these technologies into commercial processes and products, scale-up, regulatory, safety, and economic concerns are critical. © 2022 Elsevier Ltd},

note = {cited By 0},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Jafari2022,

title = {Phenolic compound profile of probiotic (Lacticaseibacillus rhamnosus LR5) fortified vegetable tablet and probiotic survival in the simulated gastrointestinal tract},

author = {S. Jafari and K. Thongmat and I. Kijpatanasilp and P. Kerdsup and P. Naknaen and M. Taweechotipatr and K. Assatarakul},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85123070698&doi=10.1038%2fs41598-022-04874-z&partnerID=40&md5=ceba471502aa4aec6701e3327c516049},

doi = {10.1038/s41598-022-04874-z},

year  = {2022},

date = {2022-01-01},

journal = {Scientific Reports},

volume = {12},

number = {1},

abstract = {The objectives of this research were to study the changes of phenolic compounds in vegetable (yellow VS green) tablets with/without probiotics (Lacticaseibacillus rhamnosus LR5) supplementation by using high performance liquid chromatography and probiotic survivability through the simulated gastrointestinal tract. The green vegetable tablets with/without probiotics had a greater (p ≤ 0.05) phenolic content compared to the yellow ones. There were no significant differences of most phenolic compound contents between probiotic-supplemented vegetable tablets and non-probiotic supplemented ones (p > 0.05). The contents of ferulic acid, epicatechin, tannic acid and rutin for both vegetable tablets tended to decrease through passing the stomach (1 and 2 h) and small intestine (2 and 4 h), however, the content of catechin in the yellow vegetable tablets tended to increase. The results also showed that the survival of Lacticaseibacillus rhamnosus LR5 slightly decreased through the simulated gastrointestinal tract. The vibrations from FTIR appeared in the wave length of 4000–3100, 3000–2800 and 1652–1545 cm−1, which accounted for the change in the N–H bonds of the amine group, changes in the structure of fatty acids and the change of carbonyl groups, respectively. This work highlighted the opportunity of application of probiotics in food products; especially non-dairy foods for consumer with dairy allergy. © 2022, The Author(s).},

note = {cited By 0},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Wongwisitchai20221096,

title = {Changes in content of bioactive constituents and antioxidant activity of riceberry after food processing and degradation kinetics during storage},

author = {S. Wongwisitchai and I. Kijpatanasilp and K. Assatarakul},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85124741156&doi=10.1111%2f1750-3841.16069&partnerID=40&md5=b31d5822d6fad5a814443c8d75857c05},

doi = {10.1111/1750-3841.16069},

year  = {2022},

date = {2022-01-01},

journal = {Journal of Food Science},

volume = {87},

number = {3},

pages = {1096-1107},

abstract = {Abstract: The effect of roasting temperature (70, 120, 140°C) and food processing (soaking, steaming, and roasting) on the content of bioactive constituents (total phenolic content, total flavonoid content, total anthocyanin, and γ-oryzanol) and antioxidant activity of processed riceberry were investigated. In addition, the degradation kinetics of bioactive constituents and antioxidant activity during storage were assessed using zero-order and first-order kinetic models. Results showed riceberry roasted at 120°C had the highest total anthocyanin content and antioxidant activity. In addition, riceberry obtained from roasting exhibited the highest bioactive compound and antioxidant activity. Besides, first-order kinetic was confirmed as the best-fitted model to describe degradation of bioactive constituents and antioxidant activity of processed riceberry during storage. This finding suggested that roasting at 120°C was chosen as an optimum condition to maximize the content of bioactive constituents and antioxidant activity and kinetic models provided a better understanding of antioxidant property reduction of processed riceberry during storage. Practical Application: Riceberry is an abundant source of bioactive constituents with beneficial health effects leading to a development of functional food product. However, processing may negatively affect biological properties of riceberry and bioactive constituent degradation of processed riceberry during storage has not been previously reported. Therefore, different processes were investigated to determine the effect on bioactive constituents and antioxidant activity of riceberry, and the degradation kinetic model of bioactive constituents during storage was also studied. Optimum processing is appropriate to design riceberry containing high concentration of bioactive constituents and antioxidant activity, which could be considered as a functional diet for health-conscious consumer. © 2022 Institute of Food Technologists®},

note = {cited By 0},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Sirichan2022,

title = {Optimization of ultrasound extraction of functional compound from makiang seed by response surface methodology and antimicrobial activity of optimized extract with its application in orange juice},

author = {T. Sirichan and I. Kijpatanasilp and N. Asadatorn and K. Assatarakul},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85123612125&doi=10.1016%2fj.ultsonch.2022.105916&partnerID=40&md5=3fb5b2e4fad2f6cc89b3ac48bb871206},

doi = {10.1016/j.ultsonch.2022.105916},

year  = {2022},

date = {2022-01-01},

journal = {Ultrasonics Sonochemistry},

volume = {83},

abstract = {An ultrasound-assisted extraction (UAE) was optimized for the extraction of bioactive compound (total phenolic compound and total flavonoid content) with antioxidant activity (DPPH and FRAP assays) using response surface methodology based on Box-Behnken design (BBD). The effect of extraction temperature (X1: 30–70 °C), extraction time (X2: 25–45 min) and amplitude (X3: 30–50%) were determined. In addition, antimicrobial activity and application of optimized makiang seed extract (MSE) were also evaluated. Results showed that the optimum condition of UAE were X1: 51.82 °C, X2: 31.87 min and X3: 40.51%. It was also found that gallic acid was the major phenolic compound of optimized MSE and its minimum inhibitiory concentration (MIC) and minimum bactericidal concentration (MBC) was between 1.56 - 6.25 and 25–100 mg/mL respectively. The addition of MSE could enhance the stability of orange juice and shelf life extension was also obtained. This research finding suggests the beneficial opportunities for ultrasound-assisted extraction for the production of bioactive compound from makiang seed with antioxidant activity leading to an application in medicinal and functional food industry. © 2022 The Authors},

note = {cited By 6},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Insang2022,

title = {Ultrasound-assisted extraction of functional compound from mulberry (Morus alba L.) leaf using response surface methodology and effect of microencapsulation by spray drying on quality of optimized extract},

author = {S. Insang and I. Kijpatanasilp and S. Jafari and K. Assatarakul},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85123351758&doi=10.1016%2fj.ultsonch.2021.105806&partnerID=40&md5=a6045ce38f886d70a2d2157510a0144f},

doi = {10.1016/j.ultsonch.2021.105806},

year  = {2022},

date = {2022-01-01},

journal = {Ultrasonics Sonochemistry},

volume = {82},

abstract = {This study aimed to optimize the ultrasound-assisted extraction (UAE) condition of mulberry leaf extract (MLE) using response surface methodology and to microencapsulate MLE by spray drying using different coating materials and ratios of coating material and MLE. The extraction results showed that MLE from condition of 60 °C (X1, temperature), 30 min (X2, time) and 60% v/v (X3, ethanol concentration) exhibited the highest bioactive compound and antioxidant activity (DPPH and FRAP assay). Based on this optimal condition, MLE was further encapsulated by spray drying. It was found that MLE encapsulated with resistant maltodextrin at ratio of MLE and resistant maltodextrin 1:1 (w/w) showed the highest encapsulation yield (%) and encapsulation efficiency (%). Water solubility, moisture content and water activity were non-significant (p > 0.05) among the microcapsules. The scanning electron microscope (SEM) revealed that the types of coating material affected their microstructures and microcapsules prepared by resistant maltodextrin as coating material had a spherical shape, smooth surface and less shrinkage than microcapsules prepared by maltodextrin and gum arabic which had rough surfaces. The highest antioxidant activity was obtained from microcapsule prepared by gum arabic at ratio of MLE and gam arabic 1:2 (w/w). In conclusion, optimal condition from UAE and encapsulation by spray drying suggest the critical potential for production of functional food with improved bioactive compound stability and maximized antioxidant activity. © 2021 The Authors},

note = {cited By 2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Nuisin2022,

title = {Facile Syntheses of Physically Crosslinked Carboxymethyl Cellulose Hydrogels and Nanocomposite Hydrogels for Enhancing Water Absorbency and Adsorption of Sappan Wood Dye},

author = {R. Nuisin and T. Siripongpreda and S. Watcharamul and K. Siralertmukul and S. Kiatkamjornwong},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85126875586&doi=10.1002%2fslct.202104598&partnerID=40&md5=e916a41241528db8a6e108b29a6b368c},

doi = {10.1002/slct.202104598},

year  = {2022},

date = {2022-01-01},

journal = {ChemistrySelect},

volume = {7},

number = {10},

abstract = {This study was focused on a facile synthesis and comprehensive characterization of carboxymethyl cellulose (CMC)-based hydrogel and their potential absorption. CMC reacted with AlIII as an ionic crosslinker to produce physical hydrogels with better gel strength but gave a low water absorption (WAC). To elucidate this question, we fabricated both the CMC hydrogel and its nanocomposite hydrogel incorporating nano-size calcium carbonate (NCC) particles using a two-step process. The AlIII- crosslinked CMC hydrogel was subsequently filled with NCC particles to give CMC/NCC nanocomposite hydrogels. The CMC/NCC1 hydrogel with NCC 1 %wv−1 gave a three-fold higher equilibrium WAC. To achieve an insight structure-property-function relation, CMC hydrogels were characterized for physically crosslinking interaction. FT-IR confirmed that AlIII ion reacts with the negative charges of carboxylate groups on the CMC chains to form the network structure via bidentate coordination. XPS data of the CMC hydrogels agreed well with the spectroscopic data. The resonance peaks of the C−O of substituent groups−CH2COO− were shifted upward to 177.9 ppm due to the incorporation of NCC. The higher chain flexibility (low tanδ) in CMC/NCC hydrogel with NCC 1 %wv−1 produced the higher WAC than did the composite hydrogel with NCC 5 %wv−1 adding. The CMC hydrogels were explored whether they are promisingly sustainable adsorbent for dye adsorption from Sappan wood extract while its CMC/NCC hydrogel rendered for better water absorption. Morphological studies revealed that NCC particles distributed mostly on the CMC surfaces to produce nanostructured composites with some particles penetrating through hydrogel pores. The specific surface areas of the CMC hydrogel increased with NCC amounts with lower WACs due to gel stiffness. The CMC hydrogels were confirmed as a promisingly sustainable adsorbent for dye adsorption from Sappan wood extract while its CMC/NCC hydrogel rendered for better water absorption which should be applicable for the dye concentration before CMC hydrogel dye adsorption. © 2022 Wiley-VCH GmbH},

note = {cited By 0},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Siralertmukul202145,

title = {Facile fabrication of chitosan/starch composite films with fumed silica as an additive},

author = {K. Siralertmukul and N. Yuenyaw and S. Watcharamul and R. Nuisin},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85116638121&doi=10.4186%2fej.2021.25.9.45&partnerID=40&md5=48907bef12d250450335caeedb80db8f},

doi = {10.4186/ej.2021.25.9.45},

year  = {2021},

date = {2021-01-01},

journal = {Engineering Journal},

volume = {25},

number = {9},

pages = {45-53},

abstract = {Chitosan/cassava starch (CS/ST) composite films with different CS:ST (w/w) ratios were prepared by solution casting and investigated as a support for fumed silica (fSiO2 ) dispersion. The CS, as a 1% (w/v) solution in 1% (w/v) acetic acid, and gelatinized ST, as a 3% (w/v) solution in 1% (w/v) acetic acid, were mixed at the appropriate volume ratios with a homogenizer, and then fSiO2 at 1, 3, or 5% (w/w of CS) was added and cast as films. The morphology, tensile strength, contact angle, X-ray diffraction (XRD) pattern, and surface functional groups of the films were investigated. The external morphology of the CS/ST composite film revealed that the fSiO2 particles were homogeneously dispersed into the polymer matrix. The water absorption and surface hydrophobicity of the composite film increased with increasing ST or CS contents. The hydrophobic acetyl groups of CS caused a notable reduction in the wettability as well as the water adsorption ability, which are preferable for a packaging film application. The CS/ST film with a high fSiO2 content had a higher tensile strength due to the reinforcement effect of the fSiO2 nanoparticles. The XRD patterns of the CS/ST films revealed that the 2θ of the crystalline region of CS were slightly changed when including the fSiO2 . The shift in the ST diffraction peak was probably due to the change in its chain orientation caused by hydrogen-bonding interactions between CS and ST molecules, resulting in their good adhesion. © 2021, Chulalongkorn University, Faculty of Fine and Applied Arts. All rights reserved.},

note = {cited By 0},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Saelim202072,

title = {Chitosan beads as the adsorbent for glyphosate removal from an aqueous solution},

author = {T. Saelim and S. Sairiam and K. Siralertmukul and S. Watcharamul and R. Nuisin},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85099903159&doi=10.14456%2fjmmm.2020.40&partnerID=40&md5=b4e8834a6206f3efd9b71b817269ad37},

doi = {10.14456/jmmm.2020.40},

year  = {2020},

date = {2020-01-01},

journal = {Journal of Metals, Materials and Minerals},

volume = {30},

number = {3},

pages = {72-82},

abstract = {Chitosan beads were prepared to remove glyphosate from an aqueous solution via a batch adsorption system. The optimum conditions, including initial glyphosate concentration, adsorbent dose, and contact time, were investigated. Increasing the initial glyphosate concentration increased the equilibrium adsorption capacity onto the chitosan beads. The rapid sorption of glyphosate was found at an initial contact time of 30 min. The maximum adsorption capacity from 35 mg• L-1 glyphosate in solution onto 0.1 g chitosan beads was 12.7 mg•g-1. Increasing amount chitosan to 0.5 g dosage could remove more than 94% of the glyphosate for all initial glyphosate concentrations (1-35 mg•L-1), pH 7, and room temperature. The pHPZC value of chitosan surface was determined approximately at pH 8. The glyphosate removal by chitosan bead was favored at pH below pHPZC. The effects on the adsorption capacity, adsorption isotherm, and kinetics model were evaluated. The glyphosate uptake behavior fitted with the Freundlich adsorption model, while the kinetic adsorption fitted a pseudo-second order. The adsorption of glyphosate was proposed to be by electrostatic interaction between the negatively charged -PO(OH)-and -COO- groups, and the positively charged –NH3+ groups of chitosan. © 2020. Journal of Metals. All rights reserved.},

note = {cited By 1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Siralertmukul2015324,

title = {Potential antibacterial activity of polystyrene nanoparticles/chitosan coated on cotton fabrics},

author = {K. Siralertmukul and S. Watcharamul and N. Wicheanpaisan and R. Nuisin},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84940030947&doi=10.1002%2fmasy.201400081&partnerID=40&md5=a2a552c42b068afae465357c78c66853},

doi = {10.1002/masy.201400081},

year  = {2015},

date = {2015-01-01},

journal = {Macromolecular Symposia},

volume = {354},

number = {1},

pages = {324-333},

abstract = {This research is to study the potential of using polystyrene nanoparticles and chitosan solution to increase the anti-bacterial activity on cotton fabrics prepared by a conventional pad-dry-cure method. Firstly, cotton fabrics were coated with chitosan solutions at the concentrations of 0.1, 0.5, and 1.0 (%w/v) and tested for the antibacterial property which was determined by clear zone measurement and viable count assay using Escherichia coli (ATCC 25922) as a tested microorganism. The results showed that, the percentage bacterial reductions were 87.2, 86.3, and 48.5%, respectively, when the cotton fabrics was coated only with chitosan solution. When polystyrene nanoparticle solution was coated onto cotton fabrics prior to coating with chitosan solutions at the concentrations of 0.1, 0.5, and 1.0 (%w/v), the percentage bacterial reductions were 90.3, 90.8, and 65.5%, respectively. This indicated that coating the cotton fabrics with polystyrene nanoparticles prior to chitosan coating would remarkably increase the antibacterial activity. The scanning electron micrographs showed that polystyrene nanoparticles were aggregated and inserted in between the interfiber spacings. The thin film of chitosan was also observed on the surface of cotton fabrics and hydrophobic property of the fabrics was tested via contact angle measurement. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.},

note = {cited By 3},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Ibrahim2022,

title = {Production and characterization of thermostable acidophilic β-mannanase from Aureobasidium pullulans NRRL 58524 and its potential in mannooligosaccharide production from spent coffee ground galactomannan},

author = {S. N. M. M. Ibrahim and W. Bankeeree and S. Prasongsuk and H. Punnapayak and P. Lotrakul},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85136473497&doi=10.1007%2fs13205-022-03301-4&partnerID=40&md5=453bcd68945062301a83bf13cfd5a2cc},

doi = {10.1007/s13205-022-03301-4},

year  = {2022},

date = {2022-01-01},

journal = {3 Biotech},

volume = {12},

number = {9},

abstract = {The maximum yield of the crude mannanase from Aureobasidium pullulans NRRL 58524 was 8.42 ± 0.18 U mL−1 when cultured for 72 h in the optimized medium containing 3% (w v−1) defatted spent coffee grounds (SCG) and 0.67% (w v−1) ammonium sulphate. Two forms of mannanase were observed in the crude enzyme and the principal mannanase was enriched to apparent homogeneity via sequential filtration and anion exchange chromatography. The molecular mass of the enzyme was approximately 63 kDa as determined by SDS–PAGE. The enriched mannanase was active at high temperatures (45–75 °C) and a pH range from 3 to 6 with the maximum activity at 55 °C and pH 4.0. The enzyme was relatively thermostable with more than 75% of its initial activity remained after a 12 h incubation at 55 °C. The half-lives of the enriched mannanase were over 8 and 6 h at 60 and 65 °C, respectively. The enzyme was not adversely affected by chelator and most ions tested. This enzyme could hydrolyze both glucomannan and galactomannan and exhibited limited catalytic activity on beta-glucan. When the crude mannanase was used to hydrolyze galactomannan extracted from SCG, the maximum yield of reducing sugars mainly comprising of mannobiose (16.27 ± 0.84 mg 100 mg−1), and mannotriose (2.85 ± 0.20 mg 100 mg−1) was obtained at 58.22 ± 2.04 mg 100 mg−1 dry weight, under optimized condition (84.87 U g−1 mannanase, 41 h 34 min incubation at 55 °C and pH 4.0). These results suggested the prospect of the enzyme in mannan hydrolysis and mannooligosaccharide production at a larger scale. © 2022, King Abdulaziz City for Science and Technology.},

note = {cited By 0},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Bankeeree20222677,

title = {Alkyl β-D-xyloside synthesis from black liquor xylan using Aureobasidium pullulans CBS 135684 β-xylosidases immobilized on spent expanded perlite},

author = {W. Bankeeree and T. Watanabe and H. Punnapayak and P. Lotrakul and S. Prasongsuk and R. Li and D. H. Y. Yanto},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85085510280&doi=10.1007%2fs13399-020-00755-5&partnerID=40&md5=1a94730d047eaf3b9268a8cd2d3b29cd},

doi = {10.1007/s13399-020-00755-5},

year  = {2022},

date = {2022-01-01},

journal = {Biomass Conversion and Biorefinery},

volume = {12},

number = {7},

pages = {2677-2686},

abstract = {The crude β-xylosidase from Aureobasidium pullulans CBS 135684 was immobilized on the surface of the modified spent expanded perlite via (3-aminopropyl) triethoxy-silane and glutaraldehyde cross-linking reaction. Over 80% of the initial free enzyme activity was detected on the bound enzyme. The immobilized β-xylosidase (204.7 ± 6.5 U g-1 of perlite) exhibited transxylosylation activity when sugars from black liquor xylan hydrolysate and a variety of alcohols (C1-6) were used as donor and acceptor molecules, respectively, yielding a number of alkyl xylosides. The synthesized hexyl xyloside exhibited some interesting properties comparable with those of a commercial hexyl glucoside, including an antioxidant activity (IC50 = 8.9 ± 2.0 mg mL-1), wetting time (43 s), and critical micelle concentration value (252 mmol L-1). Optimization for hexyl xyloside synthesis was performed via statistical analysis using a Box and Behnken design. The maximum predicted yield of hexyl xyloside (499.0 ± 1.1 mg g-1 xylan hydrolysate) was obtained with 10% (w/v) xylan hydrolysate, 10 U g-1 immobilized β-xylosidase, and 13% (v/v) hexanol at 70 °C, pH 6.0 for 4 h, and it was validated by the following experimental yield (498.9 ± 4.8 mg g-1 xylan hydrolysate). The maximum production yield remained relatively stable after the immobilized enzyme was reused for 12 cycles with a 471.5 ± 15.1 mg g-1 hexyl xyloside yield (94.4% of the first cycle). Thus, the immobilized β-xylosidase has a high potential as a robust catalyst for application in alkyl xyloside production. © 2020, Springer-Verlag GmbH Germany, part of Springer Nature.},

note = {cited By 1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Kayanna2022,

title = {Production of prebiotic aubasidan-like β-glucan from Aureobasidium thailandense NRRL 58543 and its potential as a functional food additive in gummy jelly},

author = {N. Kayanna and I. Suppavorasatit and W. Bankeeree and P. Lotrakul and H. Punnapayak and S. Prasongsuk},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85131423647&doi=10.1016%2fj.lwt.2022.113617&partnerID=40&md5=ce7568993ba6e9c898fa5bec45f239a1},

doi = {10.1016/j.lwt.2022.113617},

year  = {2022},

date = {2022-01-01},

journal = {LWT},

volume = {163},

abstract = {To enhance the yield of Aureobasidium thailandense NRRL 58543 β-glucan, effects of medium ingredients and their optimal concentrations were investigated. The highest yield of β-glucan (37.73 g/L) was obtained in the medium containing sucrose, NaNO3 and sunflower oil at concentrations of 60 g/L, 0.6 g/L, and 81.7 mL/L, respectively. The low molecular weight (LMW) β-glucan (78 kDa) was assessed its prebiotic property in comparison to the native one (>410 kDa). The growth of the probiotics, Lactobacillus casei and L. brevis, was significantly enhanced, although a higher growth was observed in the medium containing the native β-glucan. In addition, the native β-glucan was resistant with simulated salivary and gastrointestinal juice. The addition of β-glucan (2.5 and 5 g/L) in gummy jellies resulted in the increase of the color intensity, hardness, gumminess, and chewiness, while the springiness and cohesiveness decreased compared with the control. The highest score of overall acceptability (7.83 ± 0.75) according to 9-point hedonic scale was obtained from the gummy jellies with 0.5% β-glucan that could be stored at room temperature for seven days without microbial growth. Our findings suggest that A. thailandense β-glucan exhibits an immense potential in the food industry as a functional ingredient and texturing agent. © 2022 The Authors},

note = {cited By 0},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Chu2021,

title = {Starch extracted from pineapple (Ananas comosus) plant stem as a source for amino acids production},

author = {P. H. Chu and M. A. Jenol and L. Y. Phang and M. F. Ibrahim and S. Prasongsuk and W. Bankeeree and H. Punnapayak and P. Lotrakul and S. Abd-Aziz},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85108097463&doi=10.1186%2fs40538-021-00227-6&partnerID=40&md5=21f1cce269636777f02b753a61c6c93c},

doi = {10.1186/s40538-021-00227-6},

year  = {2021},

date = {2021-01-01},

journal = {Chemical and Biological Technologies in Agriculture},

volume = {8},

number = {1},

abstract = {Background: Pineapple plant (Ananas comosus) is one of the largest productions in Asia and its increasing production has generated a huge amount of pineapple wastes. Pineapple plant stem is made up of high concentration of starch which can potentially be converted into value-added products, including amino acids. Due to the increasing demand in animal feed grade amino acids, especially for methionine and lysine, the utilisation of cheap and renewable source is deemed to be an essential approach. This study aimed to produce amino acids from pineapple plant stem hydrolysates through microbial fermentation by Pediococcus acidilactici Kp10. Dextrozyme was used for hydrolysis of starch and Celluclast 1.5 L for saccharification of cellulosic materials in pineapple plant stem. Results: The hydrolysates obtained were used in the fermentation to produce methionine and lysine. Pineapple plant stem showed high starch content of 77.78%. Lignocellulosic composition of pineapple plant stem consisted of 46.15% hemicellulose, 31.86% cellulose, and 18.60% lignin. Saccharification of alkaline-treated pineapple plant stem gave lower reducing sugars of 13.28 g/L as compared to untreated, where 18.56 g/L reducing sugars obtained. Therefore, the untreated pineapple plant stem was selected for further process. Starch hydrolysis produced 57.57 g/L reducing sugar (100% hydrolysis yield) and saccharification of cellulosic materials produced 24.67 g/L reducing sugars (56.93% hydrolysis yield). The starch-based and cellulosic-based of pineapple plant stem were subjected as carbon source in methionine and lysine production by P. acidilactici Kp10. Conclusions: In conclusion, higher methionine and lysine production were produced from starch-based hydrolysis (40.25 mg/L and 0.97 g/L, respectively) as compared to cellulosic-based saccharification (37.31 mg/L and 0.84 g/L, respectively) of pineapple plant stem. Graphical Abstract: [Figure not available: see fulltext.]. © 2021, The Author(s).},

note = {cited By 0},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Bankeeree20213964,

title = {Rapid Degradation of Superabsorbent Poly(Potassium Acrylate) and its Acrylamide Copolymer Via Thermo-Oxidation by Hydrogen Peroxide},

author = {W. Bankeeree and C. Samathayanon and S. Prasongsuk and P. Lotrakul and S. Kiatkamjornwong},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85105442582&doi=10.1007%2fs10924-021-02167-6&partnerID=40&md5=cd9116d502c1cdf7b8f6a8f6be3a1fcb},

doi = {10.1007/s10924-021-02167-6},

year  = {2021},

date = {2021-01-01},

journal = {Journal of Polymers and the Environment},

volume = {29},

number = {12},

pages = {3964-3976},

abstract = {Abstract: Superabsorbent poly(potassium acrylate) (P(KA)) and poly[acrylamide-co-(potassium acrylate)] (P(Am-co-KA)) were synthesized and their effective degradation via chemical and biological processes were pursued. Significant reductions in dry mass and water absorbency were observed after P(KA) (53% and 54%, respectively) and P(Am-co-KA) (43% and 40%, respectively) were buried in the soil for ten weeks, indicating their partial biodegradation. Although the living fungal culture failed to degrade the polymers, digestion by its crude peroxidase (20 U/g, 16 h) significantly decreased the dry mass (15%; 15.0 ± 0.3 g) and water absorbency (13%; 16.0 ± 1.0 g/g) of P(KA). Chemical oxidation using H2O2 at high temperature with/without peroxidase efficiently liquidized both polymers specifying significant changes of their structures. The maximum degradation of P(KA) (99.84% weight loss) was obtained when incubated with 12.8% (v/w) H2O2 at 65 °C for 7.3 h while 98.43% weight loss was achieved after P(Am-co-KA) was incubated with 14.8% (v/w) H2O2 at 68 °C for 9.2 h. To assess the phytotoxicity of the degraded products, their effect on seed germination was determined. No significant inhibition was observed in mung bean germinating on both the untreated polymers and their oxidized products. On sweet corn, the degraded products were apparently less inhibitory than did the untreated polymers. These results suggested that the rapid and efficient degradation of polyacrylate and its copolymer by the thermo-oxidation of H2O2 could be applied for a larger scale of superabsorbent waste management. Graphic abstract: [Figure not available: see fulltext.]. © 2021, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.},

note = {cited By 2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}